Callus induction of leaf explant Piper betle L. Var Nigra with combination of plant growth regulators indole-3-acetic acid (IAA), benzyl amino purin (BAP) and kinetin

Junairiah, Nabilah Istighfari Zuraidassanaaz, Fairuz Nabil Izdihar, Yosephine Sri Wulan Manuhara

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Citations (Scopus)

Abstract

The purpose of this research was to determine the combination of plant growth regulators IAA, BAP and kinetin towards callus induction and growth of leaf explants Piper betle L. VarNigra. Explants from leaf of Piper betle L. VarNigra was cultured on MS medium with 24 treatment combinations of plant growth regulators IAA and BAP and 24 treatment combinations of plant growth regulators IAA and kinetin with 0.0;0.5;1.0;1.5;2.0 mg/L concentration respectively, the observed variable were the length of time the formation of callus, callus morphology, fresh and dry weight of callus. The results of this research showed that the combination of growth regulators IAA with BAP and kinetin had effects on leaf growth of Piper betle L. VarNigra. During 8 weeks observation, it indicated that the combination of concentration IAA 0.5 mg/L and BAP 2.0 mg/L showed fastest callus formation at 8.5 days. Combination of concentration IAA 1.0 mg/L and BAP 1.5 mg/L showed the highest of fresh weight at 0.6596 grams, and the highest dry weight was obtained from the combination of concentration IAA 0.5 mg/L and BAP 0.5 mg/L at 0.0727 grams. Combination of concentration IAA 1.0 mg/L and kinetin 1.5 mg/L had the highest of fresh weight at 0.2972 grams and the highest dry weight at 0.1660 grams. Callus of Piper betle L. VarNigra had two textures, that were compact and friable, and also showed various kind of colors, like white, greenish white, yellowish white, tanned white, brown and black. Based on this research, that concentration IAA 1.0 mg/L and 1.5 mg/L kinetin was the best combination for induction of callus from leaf of Piper betle L. Var Nigra.

Original languageEnglish
Title of host publication6th International Conference and Workshops on Basic and Applied Sciences
EditorsHewa Y. Abdullah
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735415713
DOIs
Publication statusPublished - 21 Sept 2017
Event6th International Conference and Workshops on Basic and Applied Sciences, ICOWOBAS 2017 - Erbil, Kurdistan, Iraq
Duration: 18 Mar 201719 Mar 2017

Publication series

NameAIP Conference Proceedings
Volume1888
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference6th International Conference and Workshops on Basic and Applied Sciences, ICOWOBAS 2017
Country/TerritoryIraq
CityErbil, Kurdistan
Period18/03/1719/03/17

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