The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca2+) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca2+in COCs. The role of calcium is needed for oocyte activation in the fertilization process. The purpose of this study was to measure the expression of Ca2+and the intensity of Ca2+in COCs after vitrification. The study was divided into 2 groups, the control group (C) of fresh COCs, and the treatment group (T) of COCs after vitrification. After vitrification for 24 hours, then thawing, the expression of Ca2+was examined using the Immunocytochemistry (ICC) method and the intensity of calcium (Ca2+) with a Confocal Laser Scanning Microscope (CLSM). The research data obtained were analyzed statistically by T-Test. The results showed that the expression of Ca2+in the control group (12.00±0.00) was different from the treatment group (0.35±0.79). The intensity of Ca2+in the control group (1059.43±489.59) was different from the treatment group (568.21±84.31). The conclusion of this study is that cryopreservation affects calcium in COCs; there were differences in the expression and the intensity of Ca2+between fresh COCs and COCs after vitrification. Ca2+intensity of COCs after vitrification was concentrated in the nucleus, while in fresh COCs it was concentrated in the cytoplasm.

Original languageEnglish
Pages (from-to)19-26
Number of pages8
JournalPolish Journal of Veterinary Sciences
Issue number1
Publication statusPublished - 2022


  • Calcium (Ca)
  • Kacang goat
  • confocal laser scanning microscope
  • cumulus-oocyte complex (COCs)
  • food production


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