Calcium (Ca2+) expression and intensity in cumuluc-oocyte complex(COCs) in Kacang goat after vitrification

W. Widjiati, Z. Faizah, N. Darsini, V. F. Hendrawan, H. N. Karima, C. Chotimah, S. B. Sumitro, L. R. Yustinasari, A. A.M.N. Kasman, J. M. Ntoruru, E. M. Luqman

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1 Citation (Scopus)

Abstract

The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca2+) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca2+in COCs. The role of calcium is needed for oocyte activation in the fertilization process. The purpose of this study was to measure the expression of Ca2+and the intensity of Ca2+in COCs after vitrification. The study was divided into 2 groups, the control group (C) of fresh COCs, and the treatment group (T) of COCs after vitrification. After vitrification for 24 hours, then thawing, the expression of Ca2+was examined using the Immunocytochemistry (ICC) method and the intensity of calcium (Ca2+) with a Confocal Laser Scanning Microscope (CLSM). The research data obtained were analyzed statistically by T-Test. The results showed that the expression of Ca2+in the control group (12.00±0.00) was different from the treatment group (0.35±0.79). The intensity of Ca2+in the control group (1059.43±489.59) was different from the treatment group (568.21±84.31). The conclusion of this study is that cryopreservation affects calcium in COCs; there were differences in the expression and the intensity of Ca2+between fresh COCs and COCs after vitrification. Ca2+intensity of COCs after vitrification was concentrated in the nucleus, while in fresh COCs it was concentrated in the cytoplasm.

Original languageEnglish
Pages (from-to)19-26
Number of pages8
JournalPolish Journal of Veterinary Sciences
Volume25
Issue number1
DOIs
Publication statusPublished - 2022

Keywords

  • Calcium (Ca)
  • Kacang goat
  • confocal laser scanning microscope
  • cumulus-oocyte complex (COCs)
  • food production

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