Calcium (Ca2+) expression and intensity in cumuluc-oocyte complex(COCs) in Kacang goat after vitrification

W. Widjiati; Z. Faizah; N. Darsini; V. F. Hendrawan; H. N. Karima; C. Chotimah; S. B. Sumitro; L. R. Yustinasari; A. A.M.N. Kasman; J. M. Ntoruru; E. M. Luqman

doi:10.24425/pjvs.2022.140836

Calcium (Ca²⁺) expression and intensity in cumuluc-oocyte complex(COCs) in Kacang goat after vitrification

W. Widjiati, Z. Faizah, N. Darsini, V. F. Hendrawan, H. N. Karima, C. Chotimah, S. B. Sumitro, L. R. Yustinasari, A. A.M.N. Kasman, J. M. Ntoruru, E. M. Luqman

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca²⁺) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca²⁺in COCs. The role of calcium is needed for oocyte activation in the fertilization process. The purpose of this study was to measure the expression of Ca²⁺and the intensity of Ca²⁺in COCs after vitrification. The study was divided into 2 groups, the control group (C) of fresh COCs, and the treatment group (T) of COCs after vitrification. After vitrification for 24 hours, then thawing, the expression of Ca²⁺was examined using the Immunocytochemistry (ICC) method and the intensity of calcium (Ca²⁺) with a Confocal Laser Scanning Microscope (CLSM). The research data obtained were analyzed statistically by T-Test. The results showed that the expression of Ca²⁺in the control group (12.00±0.00) was different from the treatment group (0.35±0.79). The intensity of Ca²⁺in the control group (1059.43±489.59) was different from the treatment group (568.21±84.31). The conclusion of this study is that cryopreservation affects calcium in COCs; there were differences in the expression and the intensity of Ca²⁺between fresh COCs and COCs after vitrification. Ca²⁺intensity of COCs after vitrification was concentrated in the nucleus, while in fresh COCs it was concentrated in the cytoplasm.

Original language	English
Pages (from-to)	19-26
Number of pages	8
Journal	Polish Journal of Veterinary Sciences
Volume	25
Issue number	1
DOIs	https://doi.org/10.24425/pjvs.2022.140836
Publication status	Published - 2022

Keywords

Calcium (Ca)
Kacang goat
confocal laser scanning microscope
cumulus-oocyte complex (COCs)
food production

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10.24425/pjvs.2022.140836

Cite this

Widjiati, W., Faizah, Z., Darsini, N., Hendrawan, V. F., Karima, H. N., Chotimah, C., Sumitro, S. B., Yustinasari, L. R., Kasman, A. A. M. N., Ntoruru, J. M., & Luqman, E. M. (2022). Calcium (Ca²⁺) expression and intensity in cumuluc-oocyte complex(COCs) in Kacang goat after vitrification. Polish Journal of Veterinary Sciences, 25(1), 19-26. https://doi.org/10.24425/pjvs.2022.140836

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title = "Calcium (Ca2+) expression and intensity in cumuluc-oocyte complex(COCs) in Kacang goat after vitrification",

abstract = "The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca2+) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca2+in COCs. The role of calcium is needed for oocyte activation in the fertilization process. The purpose of this study was to measure the expression of Ca2+and the intensity of Ca2+in COCs after vitrification. The study was divided into 2 groups, the control group (C) of fresh COCs, and the treatment group (T) of COCs after vitrification. After vitrification for 24 hours, then thawing, the expression of Ca2+was examined using the Immunocytochemistry (ICC) method and the intensity of calcium (Ca2+) with a Confocal Laser Scanning Microscope (CLSM). The research data obtained were analyzed statistically by T-Test. The results showed that the expression of Ca2+in the control group (12.00±0.00) was different from the treatment group (0.35±0.79). The intensity of Ca2+in the control group (1059.43±489.59) was different from the treatment group (568.21±84.31). The conclusion of this study is that cryopreservation affects calcium in COCs; there were differences in the expression and the intensity of Ca2+between fresh COCs and COCs after vitrification. Ca2+intensity of COCs after vitrification was concentrated in the nucleus, while in fresh COCs it was concentrated in the cytoplasm.",

keywords = "Calcium (Ca), Kacang goat, confocal laser scanning microscope, cumulus-oocyte complex (COCs), food production",

author = "W. Widjiati and Z. Faizah and N. Darsini and Hendrawan, {V. F.} and Karima, {H. N.} and C. Chotimah and Sumitro, {S. B.} and Yustinasari, {L. R.} and Kasman, {A. A.M.N.} and Ntoruru, {J. M.} and Luqman, {E. M.}",

note = "Funding Information: This study was supported by a Mandat Grant (SK 1408/UN3/2019) from Universitas Airlangga (Surabaya, Indonesia. Publisher Copyright: {\textcopyright} 2022 Polish Academy of Sciences. All rights reserved.",

year = "2022",

doi = "10.24425/pjvs.2022.140836",

language = "English",

volume = "25",

pages = "19--26",

journal = "Polish Journal of Veterinary Sciences",

issn = "1505-1773",

publisher = "Polish Academy of Sciences, Committee of Veterinary Sciences",

number = "1",

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TY - JOUR

T1 - Calcium (Ca2+) expression and intensity in cumuluc-oocyte complex(COCs) in Kacang goat after vitrification

AU - Widjiati, W.

AU - Faizah, Z.

AU - Darsini, N.

AU - Hendrawan, V. F.

AU - Karima, H. N.

AU - Chotimah, C.

AU - Sumitro, S. B.

AU - Yustinasari, L. R.

AU - Kasman, A. A.M.N.

AU - Ntoruru, J. M.

AU - Luqman, E. M.

N1 - Funding Information: This study was supported by a Mandat Grant (SK 1408/UN3/2019) from Universitas Airlangga (Surabaya, Indonesia. Publisher Copyright: © 2022 Polish Academy of Sciences. All rights reserved.

PY - 2022

Y1 - 2022

N2 - The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca2+) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca2+in COCs. The role of calcium is needed for oocyte activation in the fertilization process. The purpose of this study was to measure the expression of Ca2+and the intensity of Ca2+in COCs after vitrification. The study was divided into 2 groups, the control group (C) of fresh COCs, and the treatment group (T) of COCs after vitrification. After vitrification for 24 hours, then thawing, the expression of Ca2+was examined using the Immunocytochemistry (ICC) method and the intensity of calcium (Ca2+) with a Confocal Laser Scanning Microscope (CLSM). The research data obtained were analyzed statistically by T-Test. The results showed that the expression of Ca2+in the control group (12.00±0.00) was different from the treatment group (0.35±0.79). The intensity of Ca2+in the control group (1059.43±489.59) was different from the treatment group (568.21±84.31). The conclusion of this study is that cryopreservation affects calcium in COCs; there were differences in the expression and the intensity of Ca2+between fresh COCs and COCs after vitrification. Ca2+intensity of COCs after vitrification was concentrated in the nucleus, while in fresh COCs it was concentrated in the cytoplasm.

AB - The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca2+) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca2+in COCs. The role of calcium is needed for oocyte activation in the fertilization process. The purpose of this study was to measure the expression of Ca2+and the intensity of Ca2+in COCs after vitrification. The study was divided into 2 groups, the control group (C) of fresh COCs, and the treatment group (T) of COCs after vitrification. After vitrification for 24 hours, then thawing, the expression of Ca2+was examined using the Immunocytochemistry (ICC) method and the intensity of calcium (Ca2+) with a Confocal Laser Scanning Microscope (CLSM). The research data obtained were analyzed statistically by T-Test. The results showed that the expression of Ca2+in the control group (12.00±0.00) was different from the treatment group (0.35±0.79). The intensity of Ca2+in the control group (1059.43±489.59) was different from the treatment group (568.21±84.31). The conclusion of this study is that cryopreservation affects calcium in COCs; there were differences in the expression and the intensity of Ca2+between fresh COCs and COCs after vitrification. Ca2+intensity of COCs after vitrification was concentrated in the nucleus, while in fresh COCs it was concentrated in the cytoplasm.

KW - Calcium (Ca)

KW - Kacang goat

KW - confocal laser scanning microscope

KW - cumulus-oocyte complex (COCs)

KW - food production

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DO - 10.24425/pjvs.2022.140836

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