Calcium Carbonate of Blood Cockle (Anadara granosa) Shells induced VEGF-A Expression in Dentin Pulp Complex An In Vivo Study

Hermawan Adi Praja, Nina Dhaniar, Ratih Mahanani Santoso, Devy K. Putri, Annisa A.P. Salsabila, Veda A.N. Sahasika, Adioro Soetojo, Widya Saraswati

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1 Citation (Scopus)


Introduction: Mild or moderate-intensity injury will respond with a brief inflammatory response followed by reactionary dentinogenesis. Calcium hydroxide has been considered the gold standard for pulp capping materials for decades to stimulate the formation of tertiary dentin. Blood clamshells (Anadara granosa) contained a high content of calcium carbonate (CaCO3) in the blood clamshells. It is a source of calcium and it has good biocompatibility to be used as a bone repair material. This study is aimed to reveal the expression VEGF-A in odontoblast pulp cells on days 1, 3, and 7 after administration of blood clam shells derived calcium carbonate in dentin pulp complex. Methods: Thirty Wistar rats (Rattus norvegicus) were divided into 6 groups consist of 5 random samples each. The experimental group's cavity was prepared on the occlusal side of the right upper molar, and they were treated with blood clamshell calcium carbonate suspension and sealed with RMGIC. For decalcification, the solution was replaced with ethylene-diamine tetra acetic acid (EDTA) after 24 hours and was refreshed every day. Anti-VEGF-A monoclonal antibodies were used for HE and immunohistochemical staining. Result: In this study, the results were obtained consecutively on the 1st, 3rd, and 7th day with p> 0.05 for VEGF-A in all groups. Conclusion: The application of calcium carbonate is expected to be an innovative treatment in opening up new pathways for the regenerative dentin process through upregulation of VEGF-A.

Original languageEnglish
Pages (from-to)24-30
Number of pages7
JournalMalaysian Journal of Medicine and Health Sciences
Publication statusPublished - 2022


  • Anadara granosa
  • Calcium Carbonate
  • Dentin Pulp
  • Medicine
  • VEGF-A


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