TY - JOUR
T1 - Calcium Carbonate from Anadara granosa Shells Stimulates FGF2, TGF-β1, and Collagen Type 1 Expression in Rat Dental Pulp
AU - Saraswati, Widya
AU - Yahya, Azlin Noor
AU - Yonas, Yovita
AU - Cindananti, Ganiezha
AU - Rahardia, Nabiela
AU - Ramadani, Rania Rizka
AU - Ambarwati, Venny Lusanda
AU - Kusumawardhani, Devy Putri
AU - Gede Marantika Yogananda Sutela, I.
AU - Putri, Larasati Kianti
AU - Baskoro, Brian Dwi
AU - Pratama, Putu Krisnanda
AU - Setiady, Dawailatur Rahman
N1 - Publisher Copyright:
© 2025 American Medical Association. All rights reserved.
PY - 2024/12/30
Y1 - 2024/12/30
N2 - Objectives Calcium carbonate (CaCO 3), a major inorganic component in bones and teeth, offers potential protection against demineralization. This study investigates the effect of CaCO 3 from Anadara granosa shells on the expression of fibroblast growth factor 2 (FGF2), transforming growth factor-β1 (TGF-β1), and collagen type 1 in the rat dental pulp. Materials and Methods The first maxillary molars of Rattus norvegicus were perforated and subsequently pulp capped with CaCO 3 extracted from A. granosa shells. The cavities were then filled with glass ionomer cement, while the control group received calcium hydroxide (Ca(OH) 2). Teeth were extracted after 7 and 14 days of treatment, and the expression of FGF2, TGF-β1, and collagen type 1 in the dental pulp was analyzed using immunohistochemistry staining. Results The group treated with CaCO 3 from A. granosa shells exhibited significantly higher expression of FGF2, TGF-β1, and collagen type 1 in the dental pulp at both 7 and 14 days compared with the group treated with Ca(OH) 2 (p < 0.01). Conclusion The application of CaCO 3 derived from A. granosa shells enhances the proliferative phase in the dental pulp after pulp perforation and perhaps promotes reparative dentine formation.
AB - Objectives Calcium carbonate (CaCO 3), a major inorganic component in bones and teeth, offers potential protection against demineralization. This study investigates the effect of CaCO 3 from Anadara granosa shells on the expression of fibroblast growth factor 2 (FGF2), transforming growth factor-β1 (TGF-β1), and collagen type 1 in the rat dental pulp. Materials and Methods The first maxillary molars of Rattus norvegicus were perforated and subsequently pulp capped with CaCO 3 extracted from A. granosa shells. The cavities were then filled with glass ionomer cement, while the control group received calcium hydroxide (Ca(OH) 2). Teeth were extracted after 7 and 14 days of treatment, and the expression of FGF2, TGF-β1, and collagen type 1 in the dental pulp was analyzed using immunohistochemistry staining. Results The group treated with CaCO 3 from A. granosa shells exhibited significantly higher expression of FGF2, TGF-β1, and collagen type 1 in the dental pulp at both 7 and 14 days compared with the group treated with Ca(OH) 2 (p < 0.01). Conclusion The application of CaCO 3 derived from A. granosa shells enhances the proliferative phase in the dental pulp after pulp perforation and perhaps promotes reparative dentine formation.
KW - FGF2
KW - TGF-β1
KW - calcium carbonate
KW - collagen type 1
KW - dentine
KW - growth factor
UR - http://www.scopus.com/inward/record.url?scp=85214357586&partnerID=8YFLogxK
U2 - 10.1055/s-0044-1793842
DO - 10.1055/s-0044-1793842
M3 - Article
AN - SCOPUS:85214357586
SN - 1305-7456
VL - 19
SP - 811
EP - 816
JO - European Journal of Dentistry
JF - European Journal of Dentistry
IS - 3
ER -