TY - JOUR
T1 - CaCO3 from Anadara granosa shell as reparative dentin inducer in odontoblast pulp cells
T2 - In-vivo study
AU - Saraswati, Widya
AU - Soetojo, Adioro
AU - Dhaniar, Nina
AU - Praja, Hermawan Adi
AU - Santoso, Ratih Mahanani
AU - Nosla, Nadiah Salsabila
AU - Cindananti, Ganiezha
AU - Rafli, Rossabella Vennowusky
AU - Rahardia, Nabiela
N1 - Publisher Copyright:
© 2023 The Authors
PY - 2023/3/1
Y1 - 2023/3/1
N2 - Introduction: Anadara granosa (blood clam) shell contained 98.7% of calcium carbonate (CaCO3). This material has bio-properties that able to induced the dentin regeneration. This study is expected to reveal the nuclear factor kappa beta (NF-kB), transforming growth factor beta (TGF-β1), and vascular endothelial growth factor A (VEGF-A) expression in dental pulp after application of CaCO3 from Anadara granosa shell Material and methods: The thirty Rattus norvegicus strain Wistar used as model. The maxillary first molar was preparation using 0.84 mm low-speed diamond bur to made cavity. The cavity then applied glass ionomer cement (as control group) and other group applied CaCO3 from Anadara granosa shell. The teeth in each group were extracted after 1st, 3rd and 7th days of application for immunohistochemistry analysis for NF-kB, TGF-β1, and VEGF-A expression. Result: The NF-kB expression in the group with CaCO3 from Anadara granosa shell lower than control after 1st, 3rd and 7th days (p < 0.05). In other hand, the TGF-β1 and VEGF-A expression in the group with CaCO3 from Anadara granosa shell higher than control after 1st, 3rd and 7th days (p < 0.05). Conclusion: CaCO3 from Anadara granosa shell able to stimulate the TGF-β1 and VEGF-A and suppress the NF-kB expression in the dental pulp. This material able to develop as dentin-pulp material restoration.
AB - Introduction: Anadara granosa (blood clam) shell contained 98.7% of calcium carbonate (CaCO3). This material has bio-properties that able to induced the dentin regeneration. This study is expected to reveal the nuclear factor kappa beta (NF-kB), transforming growth factor beta (TGF-β1), and vascular endothelial growth factor A (VEGF-A) expression in dental pulp after application of CaCO3 from Anadara granosa shell Material and methods: The thirty Rattus norvegicus strain Wistar used as model. The maxillary first molar was preparation using 0.84 mm low-speed diamond bur to made cavity. The cavity then applied glass ionomer cement (as control group) and other group applied CaCO3 from Anadara granosa shell. The teeth in each group were extracted after 1st, 3rd and 7th days of application for immunohistochemistry analysis for NF-kB, TGF-β1, and VEGF-A expression. Result: The NF-kB expression in the group with CaCO3 from Anadara granosa shell lower than control after 1st, 3rd and 7th days (p < 0.05). In other hand, the TGF-β1 and VEGF-A expression in the group with CaCO3 from Anadara granosa shell higher than control after 1st, 3rd and 7th days (p < 0.05). Conclusion: CaCO3 from Anadara granosa shell able to stimulate the TGF-β1 and VEGF-A and suppress the NF-kB expression in the dental pulp. This material able to develop as dentin-pulp material restoration.
KW - Anadara granosa
KW - CaCO
KW - NF-kB
KW - Reparative dentin
KW - TGF-β1
KW - VEGF-A
UR - http://www.scopus.com/inward/record.url?scp=85146342915&partnerID=8YFLogxK
U2 - 10.1016/j.jobcr.2023.01.003
DO - 10.1016/j.jobcr.2023.01.003
M3 - Article
AN - SCOPUS:85146342915
SN - 2212-4268
VL - 13
SP - 164
EP - 168
JO - Journal of Oral Biology and Craniofacial Research
JF - Journal of Oral Biology and Craniofacial Research
IS - 2
ER -