TY - JOUR
T1 - Bovine hydroxyapatite extraction from cow bone waste as raw material for Bone Screw
AU - Budiatin, Aniek Setiya
AU - Hikmawati, Dyah
AU - Samirah,
AU - Aryani, Toetik
AU - Wenny, P. N.
AU - Munasir, Profinika
AU - Zaini, Oky Savitri
AU - Aprilia, Dinda Citra
N1 - Publisher Copyright:
© EM International.
PY - 2020/6
Y1 - 2020/6
N2 - This study was aimed to extract Bovine Hydroxy Apatite (BHA) from bone waste which was the sorting result of waste from the Animal Slaughtering Center, PPH. The method used was the boiling method using a high-pressure pan followed by washing with alcohol then sintered, crushed and sieved with an 80-mesh sieve. The extracted BHA was used as a bio-screw raw material by adding 10% gelatin and glutaraldehyde crosslinker. Extracted BHA was characterized by FTIR, SEM-EDAX and XRD. The results of bio-screw printing were then characterized its compressive strength. From 3.693 ± 0.264 kg of bone waste, it was obtained 1.308 ± 0.107 kg of BHA extraction result which could be calculated approximately 35.3% of the initial weight of bone waste. FTIR BHA extraction test results identified the presence of hydroxyl (OH-) functional groups at wave numbers of 570, 602, 3571 cm-1, carbonate function groups (-CO3 2-) at wave numbers of 1412, 14575 cm-1 and phosphate function groups (PO4 3-) at 473, 962,1049,1089 cm-1 wave number. SEM-EDAX test resulted particles with <1 µm size which were distributed evenly, with 1.74 Ca / P ratio. The result of X-Ray diffractometer (XRD) identified hydroxyapatite {Ca10 (PO4)5.52 (HPO4)0.15 (SiO4)0.33 (OH)1.66 O0.19} hexagonal structure with a lattice constant a = b = 9.4180Å, and c = 6.8835 Å. Bio-screw was successfully printed and tested for compressive strength which was then known that the result range from 9.56 to 11.36 MPa. The value was suitable for cancellous bone repair.
AB - This study was aimed to extract Bovine Hydroxy Apatite (BHA) from bone waste which was the sorting result of waste from the Animal Slaughtering Center, PPH. The method used was the boiling method using a high-pressure pan followed by washing with alcohol then sintered, crushed and sieved with an 80-mesh sieve. The extracted BHA was used as a bio-screw raw material by adding 10% gelatin and glutaraldehyde crosslinker. Extracted BHA was characterized by FTIR, SEM-EDAX and XRD. The results of bio-screw printing were then characterized its compressive strength. From 3.693 ± 0.264 kg of bone waste, it was obtained 1.308 ± 0.107 kg of BHA extraction result which could be calculated approximately 35.3% of the initial weight of bone waste. FTIR BHA extraction test results identified the presence of hydroxyl (OH-) functional groups at wave numbers of 570, 602, 3571 cm-1, carbonate function groups (-CO3 2-) at wave numbers of 1412, 14575 cm-1 and phosphate function groups (PO4 3-) at 473, 962,1049,1089 cm-1 wave number. SEM-EDAX test resulted particles with <1 µm size which were distributed evenly, with 1.74 Ca / P ratio. The result of X-Ray diffractometer (XRD) identified hydroxyapatite {Ca10 (PO4)5.52 (HPO4)0.15 (SiO4)0.33 (OH)1.66 O0.19} hexagonal structure with a lattice constant a = b = 9.4180Å, and c = 6.8835 Å. Bio-screw was successfully printed and tested for compressive strength which was then known that the result range from 9.56 to 11.36 MPa. The value was suitable for cancellous bone repair.
KW - Bone screw compressive strength
KW - Bone waste
KW - Bovine hydroxy Apatite
UR - http://www.scopus.com/inward/record.url?scp=85097078214&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85097078214
SN - 0971-765X
VL - 26
SP - S46-S50
JO - Ecology, Environment and Conservation
JF - Ecology, Environment and Conservation
ER -