Bone formation in rat’s calvarial defect after application of demineralized freeze dried bovine cortical bone membrane

– Bovine pericardium collagen membrane (BPCM) had been widely used in guided bone regeneration (GBR) for alveolar bone augmentation. However, it is associated with prolonged biodegradation. A newly developed Demineralized Freeze-Dried Bovine Cortical Bone Membrane (DFDBCBM) assumed to be rich in osteogenic growth factors was found to be biocompatible. Its osteogenic capacity yet to be proven. This study evaluated bone defects healing in rats’ calvarial critical sized defectafter the application of DFDBCBM and BPCM. Critical sized defect was made on calvaria bone of 30 Wistar rats. The samples were divided into 3 groups, each contains of 10 rats. The defects were then covered by DFDBCBM and BPCM in the first two groups, the control group was left to heal without membrane application. Samples were collected at 4 and 8 weeks from each group. Degree of bone healing was examined using histology examination with HE staining using bone healing score. Data was analyzed statistically with the Kruskal-Wallis test and Mann Whitney accordingly. The degree of bone healing was significantly higher in DFDBCBM groups compared to BPCM groups both in 4 and 8 weeks observation. DFDBCBM has higher capacity for bone defect healing compared to BPCM in rat’s calvaria defect.