Blood group analysis from cigarette butts by absorption inhibition method: An experimental study

Aim: In red blood cells (RBCs), antigens such as A, B, D, and H, are found to be present. The antigens are secreted in various body secretions such as semen, sweat, amniotic fluid, saliva and also blood. At the crime scene, saliva samples are very valuable, which can be collected from dry or wet cigarette butts. Blood group antigens in saliva can be found by absorption inhibition method. This study aimed to investigate blood group antigens on the cigarette butts based on time. Materials and Methods: Eighteen cigarette butt samples, used in accordance with the inclusion criteria, were placed at room temperature for 1, 3, and 6h. The estimation of blood group antigens on the cigarette butts was carried out by absorption inhibition method. Results: All samples indicated the presence of antigens in the ABO blood group. Blood group antigens in saliva on cigarette butts, after incubation from 1h until 6h, could be examined by absorption inhibition method (100%). Conclusion: Cigarette butt samples that contain saliva can be used for forensic investigations in narrowing down the suspect pool by blood group determination.