TY - JOUR
T1 - Biosurfactant production of entomopathogenic Bacillus subtilis BK7.1, as potential biocontrol bacteria, isolated from Baluran National Park, East Java, Indonesia
AU - Salamun,
AU - Susetyo, Rizky Danang
AU - Ni’matuzahroh, null
AU - Fatimah,
AU - Geraldi, Almando
AU - Supriyanto, Agus
AU - Nurhariyati, Tri
AU - Nafidiastri, Farah Aisyah
AU - Nisa’, Nabilatun
AU - Endarto,
N1 - Funding Information:
The authors would like to express appreciation for the financial support from the internal funding Featured Faculty Research (Penelitian Unggulan Fakultas) 2022-2023 and Featured Basic Research (Penelitian Dasar Unggulan) 2023 Universitas Airlangga. The authors grate full to the Dean of the Faculty of Science and Technology (Fakultas Sains dan Teknologi) and Head of Research and Community Services Institutions (Lembaga Penelitian dan Pengabdian Masyarakat), and Chancellor of Universitas Airlangga, Surabaya, Indonesia.
Publisher Copyright:
© 2023, Society for Indonesian Biodiversity. All rights reserved.
PY - 2023/3
Y1 - 2023/3
N2 - Biosurfactants as biocontrol agents have received much attention for pest control and disease vectors. The research aimed to identify the species and genetic relationship, hemolytic activity, detect coding genes, and trial production of biosurfactants on various substrates of entomopathogenic Bacillus sp. BK7.1 isolated from natural soil in Baluran National Park, East Java, Indonesia. Biosurfactant screening was carried out by testing hemolytic activity, surface tension, and emulsification activities, detecting coding genes of biosurfactant biosynthesis, and testing biosurfactant production in various substrates. The results of the molecular identification by amplifying the 16S rRNA gene using the Polymerase Chain Reaction (PCR) method for Bacillus sp. BK7.1 has a genetic similarity of 98.68% with B. subtilis subsp. inaquosorum strain BGSC 3A28. Screening showed positive hemolytic activity results, reduced surface tension, increased emulsification activities, and the production of biosurfactant in glucose, glycerol, and molasses substrates. The PCR results showed that Bacillus sp. BK7.1 had srfAA and srfAD genes encoding surfactin biosynthesis, giving it the potential to produce bioinsecticide compounds. Based on these studies, the indigenous entomopathogenic B. subtilis BK7.1 can be developed as environmentally friendly microbial bioinsecticides for pest control and disease vectors.
AB - Biosurfactants as biocontrol agents have received much attention for pest control and disease vectors. The research aimed to identify the species and genetic relationship, hemolytic activity, detect coding genes, and trial production of biosurfactants on various substrates of entomopathogenic Bacillus sp. BK7.1 isolated from natural soil in Baluran National Park, East Java, Indonesia. Biosurfactant screening was carried out by testing hemolytic activity, surface tension, and emulsification activities, detecting coding genes of biosurfactant biosynthesis, and testing biosurfactant production in various substrates. The results of the molecular identification by amplifying the 16S rRNA gene using the Polymerase Chain Reaction (PCR) method for Bacillus sp. BK7.1 has a genetic similarity of 98.68% with B. subtilis subsp. inaquosorum strain BGSC 3A28. Screening showed positive hemolytic activity results, reduced surface tension, increased emulsification activities, and the production of biosurfactant in glucose, glycerol, and molasses substrates. The PCR results showed that Bacillus sp. BK7.1 had srfAA and srfAD genes encoding surfactin biosynthesis, giving it the potential to produce bioinsecticide compounds. Based on these studies, the indigenous entomopathogenic B. subtilis BK7.1 can be developed as environmentally friendly microbial bioinsecticides for pest control and disease vectors.
KW - Bacillus subtilis BK7.1
KW - biosurfactant production
KW - crop protection
KW - entomopathogenic
KW - hemolytic activity
KW - srfAA-srfAD gene
UR - http://www.scopus.com/inward/record.url?scp=85153181858&partnerID=8YFLogxK
U2 - 10.13057/biodiv/d240353
DO - 10.13057/biodiv/d240353
M3 - Article
AN - SCOPUS:85153181858
SN - 1412-033X
VL - 24
SP - 1785
EP - 1792
JO - Biodiversitas
JF - Biodiversitas
IS - 3
ER -
