TY - JOUR
T1 - Biological Properties of Gynura procumbens Leaves Extract to MDA Levels and Antioxidant Activities in Liver of Mice
AU - Sugiharto,
AU - Wibowo, Anjar Tri
AU - Zubaidah, Ummi
AU - Savitri, Annisa Dwi
AU - Faukib, Muhammad Sidqon
AU - Salsabila, Navy Safira
AU - Manuhara, Yosephine Sri Wulan
N1 - Publisher Copyright:
© RJPT All right reserved.
PY - 2022/12
Y1 - 2022/12
N2 - The objective of our research was to the evaluated biological activities of G. procumbens leaves methanol extract (GLME) for the hepatoprotective against cadmium (Cd) toxicity in mice. Research was performed using twenty five healthy male mice, which were grouped into five treatments: P1 (control), P2 (Cd-100mg/L), P3 (GLME100mg/L+Cd-100mg/L), P4 (GLME-200mg/L+Cd-100mg/L), P5 (GLME-300mg/L+Cd-100mg/L). The results showed GLME contain phenolic and flavonoids compound by total phenolic content (TPC) and total flavonoid content (TFC) assay, they were strongly correlated with antioxidant activities. In this study, it was also known that Cd exposure increasing malondialdehyde (MDA) level and decreasing of superoxide dismutase (SOD) and catalase (CAT) activities in liver homogenates compared to control significantly. This is in line with a decreased in the number of normal cells and slightly an increased in damage cells in the histological hepatic cells. Administration of GLME can prevent liver cell damage due to Cd treatment by increasing the number of histological normal cells and the activities of SOD and CAT enzyme and reducing the level of MDA in liver homogenates. The best treatment of GLME was 100mg/L.
AB - The objective of our research was to the evaluated biological activities of G. procumbens leaves methanol extract (GLME) for the hepatoprotective against cadmium (Cd) toxicity in mice. Research was performed using twenty five healthy male mice, which were grouped into five treatments: P1 (control), P2 (Cd-100mg/L), P3 (GLME100mg/L+Cd-100mg/L), P4 (GLME-200mg/L+Cd-100mg/L), P5 (GLME-300mg/L+Cd-100mg/L). The results showed GLME contain phenolic and flavonoids compound by total phenolic content (TPC) and total flavonoid content (TFC) assay, they were strongly correlated with antioxidant activities. In this study, it was also known that Cd exposure increasing malondialdehyde (MDA) level and decreasing of superoxide dismutase (SOD) and catalase (CAT) activities in liver homogenates compared to control significantly. This is in line with a decreased in the number of normal cells and slightly an increased in damage cells in the histological hepatic cells. Administration of GLME can prevent liver cell damage due to Cd treatment by increasing the number of histological normal cells and the activities of SOD and CAT enzyme and reducing the level of MDA in liver homogenates. The best treatment of GLME was 100mg/L.
KW - Antioxidant activity
KW - Gynura procumbens
KW - Health care
KW - Hepatoprotective
KW - MDA
UR - http://www.scopus.com/inward/record.url?scp=85146278529&partnerID=8YFLogxK
U2 - 10.52711/0974-360X.2022.00984
DO - 10.52711/0974-360X.2022.00984
M3 - Article
AN - SCOPUS:85146278529
SN - 0974-3618
VL - 15
SP - 5829
EP - 5834
JO - Research Journal of Pharmacy and Technology
JF - Research Journal of Pharmacy and Technology
IS - 12
ER -