TY - JOUR
T1 - Biological hazard on multidrug resistance (MDR) of escherichia coli collected from cloacal swab of broiler chicken on wet markets Surabaya
AU - Harijani, Nenny
AU - Oetama, Surya Jovinson Tansil
AU - Soepranianondo, Koesnoto
AU - Effendi, Mustofa Helmi
AU - Tyasningsih, Wiwiek
N1 - Publisher Copyright:
© 2020, Institute of Medico-Legal Publications. All rights reserved.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Objective: The purpose of this study was to determine the multidrug resistance (MDR) profile of Escherichia coli from broiler chicken from Wonokromo Market and Tambahrejo Market Surabaya to some antibiotic groups, namely streptomycin, levofloxacin, cefotaxime, trimethoprim and chloramphenicol. Materials and Methods: Seventy samples were taken from Wonokromo market and Tambahrejo market, each of which had thirty-five samples. Sampling was used cloaca swab technique. Then inoculated on Eosin Methylene Blue Agar (EMBA) media, purification of bacteria on EMBA media, and tested biochemically with Sulfide Indol Motility (SIM) and Triple Sugar Iron Agar (TSIA) media. Antibiotic sensitivity test was used the Kirby-Bauer method. Muller-Hinton Agar (MHA) media incubation. Then the inhibition zone was measured according to the Clinical and Labortary Standard Institute (CLSI) standards. Detect positive multidrug resistance (MDR) bacteria characterized by resistance to ≥3 types of antibiotics. Results: Detection results of Escherichia coli multidrug resistance (MDR) at Wonokromo market was 85.7%, and at Tambahrejo market was 51.4%. Presumptive Extended Spectrum Beta Lactamase (ESBL) producing E. coli at Wonokromo market was 14.3% and at Tambahrejo market was 2.9%. Conclusion: The high level of MDR of Escherichia coli from cloacal swabs of broiler chicken was a threat to public health and the environment, and is an important concern to reduce the rate of its spread.
AB - Objective: The purpose of this study was to determine the multidrug resistance (MDR) profile of Escherichia coli from broiler chicken from Wonokromo Market and Tambahrejo Market Surabaya to some antibiotic groups, namely streptomycin, levofloxacin, cefotaxime, trimethoprim and chloramphenicol. Materials and Methods: Seventy samples were taken from Wonokromo market and Tambahrejo market, each of which had thirty-five samples. Sampling was used cloaca swab technique. Then inoculated on Eosin Methylene Blue Agar (EMBA) media, purification of bacteria on EMBA media, and tested biochemically with Sulfide Indol Motility (SIM) and Triple Sugar Iron Agar (TSIA) media. Antibiotic sensitivity test was used the Kirby-Bauer method. Muller-Hinton Agar (MHA) media incubation. Then the inhibition zone was measured according to the Clinical and Labortary Standard Institute (CLSI) standards. Detect positive multidrug resistance (MDR) bacteria characterized by resistance to ≥3 types of antibiotics. Results: Detection results of Escherichia coli multidrug resistance (MDR) at Wonokromo market was 85.7%, and at Tambahrejo market was 51.4%. Presumptive Extended Spectrum Beta Lactamase (ESBL) producing E. coli at Wonokromo market was 14.3% and at Tambahrejo market was 2.9%. Conclusion: The high level of MDR of Escherichia coli from cloacal swabs of broiler chicken was a threat to public health and the environment, and is an important concern to reduce the rate of its spread.
KW - Broiler chicken
KW - Cloacal swab
KW - Escherichia coli
KW - Multidrug resistance
KW - Public health
UR - http://www.scopus.com/inward/record.url?scp=85099333334&partnerID=8YFLogxK
U2 - 10.37506/ijfmt.v14i4.12125
DO - 10.37506/ijfmt.v14i4.12125
M3 - Article
AN - SCOPUS:85099333334
SN - 0973-9122
VL - 14
SP - 3239
EP - 3244
JO - Indian Journal of Forensic Medicine and Toxicology
JF - Indian Journal of Forensic Medicine and Toxicology
IS - 4
ER -