Biological hazard on multidrug resistance (MDR) of escherichia coli collected from cloacal swab of broiler chicken on wet markets Surabaya

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Abstract

Objective: The purpose of this study was to determine the multidrug resistance (MDR) profile of Escherichia coli from broiler chicken from Wonokromo Market and Tambahrejo Market Surabaya to some antibiotic groups, namely streptomycin, levofloxacin, cefotaxime, trimethoprim and chloramphenicol. Materials and Methods: Seventy samples were taken from Wonokromo market and Tambahrejo market, each of which had thirty-five samples. Sampling was used cloaca swab technique. Then inoculated on Eosin Methylene Blue Agar (EMBA) media, purification of bacteria on EMBA media, and tested biochemically with Sulfide Indol Motility (SIM) and Triple Sugar Iron Agar (TSIA) media. Antibiotic sensitivity test was used the Kirby-Bauer method. Muller-Hinton Agar (MHA) media incubation. Then the inhibition zone was measured according to the Clinical and Labortary Standard Institute (CLSI) standards. Detect positive multidrug resistance (MDR) bacteria characterized by resistance to ≥3 types of antibiotics. Results: Detection results of Escherichia coli multidrug resistance (MDR) at Wonokromo market was 85.7%, and at Tambahrejo market was 51.4%. Presumptive Extended Spectrum Beta Lactamase (ESBL) producing E. coli at Wonokromo market was 14.3% and at Tambahrejo market was 2.9%. Conclusion: The high level of MDR of Escherichia coli from cloacal swabs of broiler chicken was a threat to public health and the environment, and is an important concern to reduce the rate of its spread.

Original languageEnglish
Pages (from-to)3239-3244
Number of pages6
JournalIndian Journal of Forensic Medicine and Toxicology
Volume14
Issue number4
DOIs
Publication statusPublished - 1 Oct 2020

Keywords

  • Broiler chicken
  • Cloacal swab
  • Escherichia coli
  • Multidrug resistance
  • Public health

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