Biodegradation study of demineralized freeze dried bovine cortical bone membrane after subcutaneous implantation in rat’s dorsum

Astrid B.U. Purba, David B. Kamadjaja, Akhsanal Fauzi, Ikhram Kharis, Andra Rizqiawan, Coen Pramono

Research output: Contribution to journalArticlepeer-review

Abstract

– Guided bone regeneration (GBR) for alveolar bone augmentation commonly uses collagen membrane made from bovine pericardium membrane (BPCM). However, it has been associated with prolonged biodegradation. Demineralized freeze dried bovine cortical bone membrane (DFDBCBM) is a newly developed membrane to be used as an alternative to BPCM. As BCBM is composed of native collagen tissue; it may undergo early degradation which is not appropriate for barrier membrane. This study aims to determine biodegradation rates of DFDBCBM compared with that of BPCM after subcutaneous implantation in rat’s dorsum. Forty eight rats used as samples were divided into 2 groups (DFDBCBM and BPCM). Six samples from each group were sacrificed after 7, 14, 21, and 28 days of subcutaneous implantation. The specimens were processed and stained with HE for histology examination. Study of biodegradation rate membrane was done by measuring the thickness of residual membrane using light microscope. The result of study showed that there were no significant difference in biodegradation rates between DFDBCBM compared to BPCM after implantation in all observation periods (p>0,05). We conclude that demineralized freeze dried bovine cortical bone membrane has appropriate biodegradation rate, therefore it is potential to be used as guided bone regeneration membrane.

Original languageEnglish
Pages (from-to)S93-S96
JournalAsian Journal of Microbiology, Biotechnology and Environmental Sciences
Volume20
Issue numberDecember
Publication statusPublished - 2018

Keywords

  • Biodegradation rates
  • Freeze dried demineralized bovine cortical bone membrane
  • Guided bone regeneration

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