Bioanalytical method development and validation for quantification of morachalcone A in rabbit plasma using high performance liquid chromatography

Artocarpus champeden (A. champeden) ethanol extract has been reported as antimalarial activity and prospective to be developed as phytomedicine products. The active marker compound was identical with known prenylated chalcone compound, Morachalcone A. To further develop phytomedicine products from A. champeden especially in aspects of bioavailability and pharmacokinetic, a valid, selective and sensitive analytical method becomes important to determine morachalcone A in plasma. The aim of study was to develop and validate selectivity and sensitivity of High Performance Liquid Chromatography (HPLC) method to determine morachalcone A in rabbit plasma. This method was used a RP-18 Column (250 x 4.6 mm i.d, 5 µm), under isocratic elution and acetonitrile:water (50:50 v/v) was used as mobile phase with flow rate of 1.0ml/min. Detection was carried out at 368 nm, 4-hydroxychalcone and methanol were used as internal standard and precipitant. Results showed that this HPLC method was selective with good linearity in range of 3096.774 to 154.839ng/ml. LOD and LLOQ were 89.384 and 154.839ng/ml, respectively. The mean %different was found between 2.79 to 14.33%. Intra and inter-day precision were ≤15% and recovery from this extraction method of morachalcone A and Internal Standard were 80-120%.