TY - JOUR
T1 - Beneficial Effect of Resveratrol on Conditioned Medium Human Adipose-derived Mesenchymal Stem Cell for Cutaneous Wound Healing Related Growth Factors
AU - Alinda, Medhi Denisa
AU - Listiawan, Muhammad Yulianto
AU - Endaryanto, Anang
AU - Suroto, Heri
AU - Komaratih, Evelyn
AU - Rantam, Fedik Abdul
AU - Kusumaputra, Bagus Haryo
AU - Agusni, Regitta Indira
AU - Rindiastuti, Yuyun
AU - Prakoeswa, Camilla Amanda
AU - Sigit Prakoeswa, Cita Rosita
N1 - Publisher Copyright:
© 2021 UPM Press. All rights reserved.
PY - 2021/9
Y1 - 2021/9
N2 - Introduction: Cutaneous wound healing, a type of soft tissue injury, needs the interaction between multiple growth factors (epidermal growth factor [EGF], transforming growth factor [TGF], platelet-derived growth factor [PDGF], and vascular endothelial growth factor [VEGF]) in order to attain physiological healing. Nevertheless, in certain conditions, i.e., chronic wound, certain growth factors might be insufficient. VEGF is among the most significant pro-angiogenic mediators during wound healing to increase migration and invasion of endothelial cells. Conditioned medium adipose-derived mesenchymal stem cells (CM-AdMSCs) are a new hope to ameliorate impaired healing. Resveratrol (RV), a small molecule that can increase the effect of CM-AdMSCs, is needed to help stimulates growth factors from stem cells for culture. This study aims to evaluate growth factors in CM-AdMSCs with resveratrol. Methods: Isolated and characterized MSCs from CM-AdMSCs treated with RV supplemented Serum Deprived Medium (SDM) were used in this study. Growth factors level were measured using ELISA. Results: A decrease of mean were noted in EGF (290.19±53.19 VS 271.64±52.58) and VEGF (788.76±204.82 VS 117.92±49.22), while an increase of mean in TGF (633.14±11.38 VS 824.73±121.15) and PDGF (2999.00±203.54 VS 3961.40±378.68) between CM-AdMSCs without resveratrol and with resveratrol group, respectively. PDGF and VEGF were statistically significant (p < 0.05). Conclusion: In conclusion, RV promotes PDGF secretion and decreases VEGF in CM-AdMSCs.
AB - Introduction: Cutaneous wound healing, a type of soft tissue injury, needs the interaction between multiple growth factors (epidermal growth factor [EGF], transforming growth factor [TGF], platelet-derived growth factor [PDGF], and vascular endothelial growth factor [VEGF]) in order to attain physiological healing. Nevertheless, in certain conditions, i.e., chronic wound, certain growth factors might be insufficient. VEGF is among the most significant pro-angiogenic mediators during wound healing to increase migration and invasion of endothelial cells. Conditioned medium adipose-derived mesenchymal stem cells (CM-AdMSCs) are a new hope to ameliorate impaired healing. Resveratrol (RV), a small molecule that can increase the effect of CM-AdMSCs, is needed to help stimulates growth factors from stem cells for culture. This study aims to evaluate growth factors in CM-AdMSCs with resveratrol. Methods: Isolated and characterized MSCs from CM-AdMSCs treated with RV supplemented Serum Deprived Medium (SDM) were used in this study. Growth factors level were measured using ELISA. Results: A decrease of mean were noted in EGF (290.19±53.19 VS 271.64±52.58) and VEGF (788.76±204.82 VS 117.92±49.22), while an increase of mean in TGF (633.14±11.38 VS 824.73±121.15) and PDGF (2999.00±203.54 VS 3961.40±378.68) between CM-AdMSCs without resveratrol and with resveratrol group, respectively. PDGF and VEGF were statistically significant (p < 0.05). Conclusion: In conclusion, RV promotes PDGF secretion and decreases VEGF in CM-AdMSCs.
KW - CM-AdMSCs
KW - PDGF
KW - Resveratrol
KW - VEGF
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=85117123320&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85117123320
SN - 1675-8544
VL - 17
SP - 11
EP - 16
JO - Malaysian Journal of Medicine and Health Sciences
JF - Malaysian Journal of Medicine and Health Sciences
ER -