TY - JOUR
T1 - Basic Fibroblast Growth Factor Expression after Gingival Mesenchymal Stem Cell’s Metabolite Provision in Lipopolysaccharide induce inflammatory bone resorption in vivo
AU - Rahmawati, Dwi
AU - Nugraha, Alexander Patera
AU - Saraya, Alivianda Zahrina
AU - Ramadhani, Nastiti Faradilla
AU - Riawan, Wibi
AU - Ihsan, Igo Syaiful
AU - Ernawati, Diah Savitri
AU - Narmada, Ida Bagus
AU - Saskianti, Tania
AU - Rezkita, Fianza
AU - Sarasati, Andari
AU - Nugraha, Albertus Putera
AU - Binti, Tengku Natasha Eleena
AU - Noor, Tengku Ahmad
N1 - Funding Information:
The authors are grateful to the authorities of Stem Cells Research and Development Center; Faculty of Dental Medicine Research Center, Universitas Airlangga, Surabaya, East Java, Indonesia for the support and the facilities. This study was supported by Hibah Internal Penelitian Dosen Pemula (PDP) 2021 with appointment number 212/UN3/2021 Universitas Airlangga, Surabaya, East Java, Indonesia
Publisher Copyright:
© 2022. Journal of International Dental and Medical Research. All Rights Reserved.
PY - 2022
Y1 - 2022
N2 - balanced. Some chronic disease, such us periodontitis could affect the bone remodelling causing bone resorption is higher than bone formation. Basic Fibroblast Growth Factors (bFGF) has role on osteogenesis which can help in increasing bone formation. Gingival Mesenchymal Stem Cells’s Metabolite (GM SCM) is medical wasted products from Mesenchymal Stem Cells (MSC) which has various function. Aim of this study is to investigate the metabolites of GM SC effect on bFGF expression in inflammatory bone resorption caused by lipopolysaccharide. The 20 experimental animals were separated into four groups: control (C): 100 g PBS day 1-7, LPS group: 100 g LPS day 1-7, LPS+GM SCs' metabolite group: 100 g LPS + 100 g GM SCs' metabolite day 1-1-7, and GM SCs' metabolite group: 100 g M-GM SCs day 1-7. Escherichia Coli LPS was employed to trigger bone resorption on the calvaria of an animal model. The dose of GM SCs metabolite administered is 100 g once day through subcutaneous injection. Furthermore, on day 8, all samples were sacrificed by cervical dislocation. To count the number of bFGF positive expressions in osteoblast in the calvaria of animal models, bFGF monoclonal antibody and Diaminobenzidine (DAB) is added, resulting in a brown precipitate developing where the antibody has attached. The statistical analysis was performed to examine the significantly different between groups (p<0.05). The expression of bFGF was significantly decreased in LPS induced bone resorption group (LPS group), however, after GM SCs’ metabolite provision, bFGF expression was significantly elevated in GMSCs metabolite and LPS induced bone resorption (LPS+GM SCs’ metabolite group) with significantly different (p=0.0001; p<0.05). The positive expression of bFGF in osteoblast was elevated after GM SCs metabolite provision in LPS-induced calvaria bone resorption in wistar rats (R. novergicus) by means of immunohistochemistry examination.
AB - balanced. Some chronic disease, such us periodontitis could affect the bone remodelling causing bone resorption is higher than bone formation. Basic Fibroblast Growth Factors (bFGF) has role on osteogenesis which can help in increasing bone formation. Gingival Mesenchymal Stem Cells’s Metabolite (GM SCM) is medical wasted products from Mesenchymal Stem Cells (MSC) which has various function. Aim of this study is to investigate the metabolites of GM SC effect on bFGF expression in inflammatory bone resorption caused by lipopolysaccharide. The 20 experimental animals were separated into four groups: control (C): 100 g PBS day 1-7, LPS group: 100 g LPS day 1-7, LPS+GM SCs' metabolite group: 100 g LPS + 100 g GM SCs' metabolite day 1-1-7, and GM SCs' metabolite group: 100 g M-GM SCs day 1-7. Escherichia Coli LPS was employed to trigger bone resorption on the calvaria of an animal model. The dose of GM SCs metabolite administered is 100 g once day through subcutaneous injection. Furthermore, on day 8, all samples were sacrificed by cervical dislocation. To count the number of bFGF positive expressions in osteoblast in the calvaria of animal models, bFGF monoclonal antibody and Diaminobenzidine (DAB) is added, resulting in a brown precipitate developing where the antibody has attached. The statistical analysis was performed to examine the significantly different between groups (p<0.05). The expression of bFGF was significantly decreased in LPS induced bone resorption group (LPS group), however, after GM SCs’ metabolite provision, bFGF expression was significantly elevated in GMSCs metabolite and LPS induced bone resorption (LPS+GM SCs’ metabolite group) with significantly different (p=0.0001; p<0.05). The positive expression of bFGF in osteoblast was elevated after GM SCs metabolite provision in LPS-induced calvaria bone resorption in wistar rats (R. novergicus) by means of immunohistochemistry examination.
KW - Dentistry
KW - Gingival mesenchymal stem cells metabolite
KW - Infectious disease
KW - Lipopolysaccharide
KW - Medicine
UR - http://www.scopus.com/inward/record.url?scp=85133733104&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85133733104
SN - 1309-100X
VL - 15
SP - 468
EP - 471
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 2
ER -