Automatic system for stitching microscopic images using OpenPano

Imas Sukaesih Sitanggang, Auriza Akbar, Riries Rulaningtyas, Andriyan Bayu Suksmono, Kuwat Triyana, Dwi Winarni

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)


Analyzing pap smear images by manual reading through a microscope is a challenge for doctors especially in distinguishing epithelial cells from inflammatory cells. A computer-based application is required for cervical cancer screening and displaying the results of reading automatically pap smear images using a microscope. This study aims to develop a module for stitching microscopic images including pap smear images which are collected from a microscope. A module for automatically stitching pap smear images is an important component in pap smear images management system. There are three tasks in stitching several pap smear images which were implemented in this study. Those three tasks include 1) feature extraction with scale-invariant feature transform, 2) estimation of homography or affine transformation by random sample consensus and 3) optimization of connection between images with bundle adjustment and blending. We use OpenPano to implement those three tasks. The system has successfully stitched the pap smear images with the feature overlap among the images greater than 10%. The stitched pap smear image has good quality after smoothing using the vertical blending step. In addition, blurry pixels on in some spots disappeared after performing the stitching error. The image quality has been improved after the multiband blending using 16-band and 128-band which shows the significant improvement on the 128-band. The system is expected to assist doctors in pap smear image identification.

Original languageEnglish
Title of host publication2nd International Conference on Physical Instrumentation and Advanced Materials 2019
EditorsHerri Trilaksana, Sulaiman Wadi Harun, Cameron Shearer, Moh Yasin
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735440562
Publication statusPublished - 9 Dec 2020
Event2nd International Conference on Physical Instrumentation and Advanced Materials, ICPIAM 2019 - Surabaya, Indonesia
Duration: 22 Oct 2019 → …

Publication series

NameAIP Conference Proceedings
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616


Conference2nd International Conference on Physical Instrumentation and Advanced Materials, ICPIAM 2019
Period22/10/19 → …


Dive into the research topics of 'Automatic system for stitching microscopic images using OpenPano'. Together they form a unique fingerprint.

Cite this