TY - JOUR
T1 - Auto-regeneration of mice testicle seminiferous tubules due to malnutrition based on stem cells mobilization using honey
AU - Safitri, Erma
AU - Utama, Suzanita
AU - Widiyatno, Thomas Valentinus
AU - Sandhika, Willy
AU - Prasetyo, R. Heru
N1 - Publisher Copyright:
© 2015 The Authors.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Objective: To find out a novel therapy based on mobilization and differentiation of own body-derived stem cells using natural bee honey. Methods: Testicle degeneration model of mouse is available through food fasting 5 days long that causes malnutrition and affects the testicle. Then administration of 30% (T1) and 50% bee honey (T2) each for five days long and compared to the positive control, fast without bee honey (T0+) and negative control, feed but without bee honey (T0-). Subsequently observation of Hematopoietic Stem Cells (HSCs) mobilization according to the CD34 and CD45 expressions performed using flowcytometry method was conducted. Besides, identification of differentiation is examined through the expression of spermatogonial stem cells (SSCs) using immunohistochemistry technique, while the seminiferous tubules cell regeneration through H&E staining microscopic examination. Results: Stem cells mobilization based on the expression of CD34 and CD45, which is a marker of HSCs. Differentiation of stem cells into progenitor cells that expected based expression of SSCs in testicle tissue. SSCs increase causes regeneration testicle seminiferous tubules. Conclusions: Results of this study revealed a significantly different of C34 and CD45 expressions between groups, also an increase SSCs expression and testicle seminiferous tubules cells regeneration as well.
AB - Objective: To find out a novel therapy based on mobilization and differentiation of own body-derived stem cells using natural bee honey. Methods: Testicle degeneration model of mouse is available through food fasting 5 days long that causes malnutrition and affects the testicle. Then administration of 30% (T1) and 50% bee honey (T2) each for five days long and compared to the positive control, fast without bee honey (T0+) and negative control, feed but without bee honey (T0-). Subsequently observation of Hematopoietic Stem Cells (HSCs) mobilization according to the CD34 and CD45 expressions performed using flowcytometry method was conducted. Besides, identification of differentiation is examined through the expression of spermatogonial stem cells (SSCs) using immunohistochemistry technique, while the seminiferous tubules cell regeneration through H&E staining microscopic examination. Results: Stem cells mobilization based on the expression of CD34 and CD45, which is a marker of HSCs. Differentiation of stem cells into progenitor cells that expected based expression of SSCs in testicle tissue. SSCs increase causes regeneration testicle seminiferous tubules. Conclusions: Results of this study revealed a significantly different of C34 and CD45 expressions between groups, also an increase SSCs expression and testicle seminiferous tubules cells regeneration as well.
KW - Bee honey
KW - Malnutrition
KW - Seminiferous tubule auto-regeneration
KW - Stem cell mobilization
UR - http://www.scopus.com/inward/record.url?scp=84965170471&partnerID=8YFLogxK
U2 - 10.1016/j.apjr.2015.12.005
DO - 10.1016/j.apjr.2015.12.005
M3 - Article
AN - SCOPUS:84965170471
SN - 2305-0500
VL - 5
SP - 31
EP - 35
JO - Asian Pacific Journal of Reproduction
JF - Asian Pacific Journal of Reproduction
IS - 1
ER -
