TY - JOUR
T1 - Atopic biomarker changes after exposure to Porphyromonas gingivalis lipopolysaccharide
T2 - a small experimental study in Wistar rats [version 1; peer review: awaiting peer review]
AU - Nelwan, Sindy Cornelia
AU - Nugraha, Ricardo Adrian
AU - Endaryanto, Anang
AU - Meizarini, Asti
AU - Tedjosasongko, Udijanto
AU - Pradopo, Seno
AU - Utomo, Haryono
AU - Nowwarote, Nunthawan
N1 - Funding Information:
This project is fully supported by Agung Sosiawan as Dean of Universitas Airlangga College of Dentistry and Coen Pramono as Director of Airlangga Oral and Dental Hospital. We would like to acknowledge Harjanto from Physiology Department and Indrawati Retno from Biochemistry Department for providing material and reagent generation. We also thank all lecturers and research assistants from Universitas Airlangga, who are willing to help in the technical aspect
Publisher Copyright:
© 2021 Nelwan SC et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
PY - 2021
Y1 - 2021
N2 - Background: IgE and IgG4 are implicated in atopic development and clinically utilized as major biomarkers. Atopic responses following certain pathogens, such as Porphyromonas gingivalis (Pg), are currently an area of interest for further research. The aim of this study is to measure the level of IgE, IgG4, and IgG4/IgE ratio periodically after exposure of periodontal pathogen Pg lipopolysaccharide (LPS). Methods: We used 16 Wistar rats (Rattus norvegicus) randomly subdivided into four groups: Group 1, injected with placebo; Group 2, injected with LPS Pg 0.3 μg/mL; Group 3, injected with LPS Pg 1 μg/mL; and Group 4, injected with LPS Pg 3 μg/mL. Sera from all groups were taken from retro-orbital plexus before and after exposure. Results: Levels of IgE and IgG4 increased significantly following exposure of LPS Pg at day-4 and day-11. Greater increase of IgE rather than IgG4 contributed to rapid decline of IgG4/IgE ratio, detected in the peripheral blood at day-4 and day-11. Conclusion: Modulation of atopic responses following exposure to LPS Pg is reflected by a decrease in IgG4/IgE ratio that accompanies an increase of IgE. Therefore, Pg, a keystone pathogen during periodontal disease, may have a tendency to disrupt atopic biomarkers.
AB - Background: IgE and IgG4 are implicated in atopic development and clinically utilized as major biomarkers. Atopic responses following certain pathogens, such as Porphyromonas gingivalis (Pg), are currently an area of interest for further research. The aim of this study is to measure the level of IgE, IgG4, and IgG4/IgE ratio periodically after exposure of periodontal pathogen Pg lipopolysaccharide (LPS). Methods: We used 16 Wistar rats (Rattus norvegicus) randomly subdivided into four groups: Group 1, injected with placebo; Group 2, injected with LPS Pg 0.3 μg/mL; Group 3, injected with LPS Pg 1 μg/mL; and Group 4, injected with LPS Pg 3 μg/mL. Sera from all groups were taken from retro-orbital plexus before and after exposure. Results: Levels of IgE and IgG4 increased significantly following exposure of LPS Pg at day-4 and day-11. Greater increase of IgE rather than IgG4 contributed to rapid decline of IgG4/IgE ratio, detected in the peripheral blood at day-4 and day-11. Conclusion: Modulation of atopic responses following exposure to LPS Pg is reflected by a decrease in IgG4/IgE ratio that accompanies an increase of IgE. Therefore, Pg, a keystone pathogen during periodontal disease, may have a tendency to disrupt atopic biomarkers.
KW - Porphyromonas gingivalis LPS
KW - allergic diseases
KW - atopic inflammatory pathway
KW - immunoglobulin
KW - periodontal pathogen
UR - http://www.scopus.com/inward/record.url?scp=85113890807&partnerID=8YFLogxK
U2 - 10.12688/f1000research.51959.1
DO - 10.12688/f1000research.51959.1
M3 - Article
AN - SCOPUS:85113890807
SN - 2046-1402
VL - 10
SP - 1
EP - 13
JO - F1000Research
JF - F1000Research
ER -