TY - JOUR
T1 - Association between reversine dose and increased plasticity of dedifferentiated fat (DFAT cells) into cardiac derived cells
AU - Ramadhan, Muhammad Firdani
AU - Oktaviono, Yudi Her
AU - Dharmadjati, Budi Baktijasa
AU - Intan, Ryan Enast
N1 - Publisher Copyright:
© 2022 Czech Society of Cardiology Z.S. All rights reserved.
PY - 2022/12
Y1 - 2022/12
N2 - Aim: To analyze the association between reversine and increased plasticity of DFAT into cardiac derivative cells. Method: The cultured DFAT cells were divided into four groups based on reversine dose: control (no reversine), 10 nM, 20 nM, and 40 nM reversine. Each group will go through several stages of passage before further differentiation into cardiomyocytes (marked by cTnT expression), VSCMs (marked by alpha-SMA expression), and vascular endothelial cells (marked by alpha-SMA expression) (marked by CD31 expression). Result: There were signifi cant differences in the expression of cTnT, alpha-SMA, and CD31 (p = 0.003, p <0.001, and p <0.001, respectively) in each group of DFAT cells that received reversine. From post-hoc analysis with Tukey test, it was found that only the 10 nM reversine group produced a signifi cant difference compared to the control group (p = 0.002) for cTnT expression and reversine 10 nM and 20 nM group for -SMA expression and CD31 expression (p = 0.028 and p <0.001, respectively). Conclusions: This study proves that there is a relationship between reversine and increased plasticity of DFAT cells into cardiac derived cells in the form of cardiomyocytes (cTnT), VSMCs (alpha-SMA), and vascular endothelial cells (CD31).
AB - Aim: To analyze the association between reversine and increased plasticity of DFAT into cardiac derivative cells. Method: The cultured DFAT cells were divided into four groups based on reversine dose: control (no reversine), 10 nM, 20 nM, and 40 nM reversine. Each group will go through several stages of passage before further differentiation into cardiomyocytes (marked by cTnT expression), VSCMs (marked by alpha-SMA expression), and vascular endothelial cells (marked by alpha-SMA expression) (marked by CD31 expression). Result: There were signifi cant differences in the expression of cTnT, alpha-SMA, and CD31 (p = 0.003, p <0.001, and p <0.001, respectively) in each group of DFAT cells that received reversine. From post-hoc analysis with Tukey test, it was found that only the 10 nM reversine group produced a signifi cant difference compared to the control group (p = 0.002) for cTnT expression and reversine 10 nM and 20 nM group for -SMA expression and CD31 expression (p = 0.028 and p <0.001, respectively). Conclusions: This study proves that there is a relationship between reversine and increased plasticity of DFAT cells into cardiac derived cells in the form of cardiomyocytes (cTnT), VSMCs (alpha-SMA), and vascular endothelial cells (CD31).
KW - Cardiomyocyte
KW - DFAT
KW - Reversine
KW - VSMCs
KW - Vascular endothelial cells
UR - http://www.scopus.com/inward/record.url?scp=85147340899&partnerID=8YFLogxK
U2 - 10.33678/cor.2022.033
DO - 10.33678/cor.2022.033
M3 - Article
AN - SCOPUS:85147340899
SN - 0010-8650
VL - 64
SP - 589
EP - 594
JO - Cor et Vasa
JF - Cor et Vasa
IS - 6
ER -