Association between reversine dose and increased plasticity of dedifferentiated fat (DFAT cells) into cardiac derived cells

Muhammad Firdani Ramadhan, Yudi Her Oktaviono, Budi Baktijasa Dharmadjati, Ryan Enast Intan

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Aim: To analyze the association between reversine and increased plasticity of DFAT into cardiac derivative cells. Method: The cultured DFAT cells were divided into four groups based on reversine dose: control (no reversine), 10 nM, 20 nM, and 40 nM reversine. Each group will go through several stages of passage before further differentiation into cardiomyocytes (marked by cTnT expression), VSCMs (marked by alpha-SMA expression), and vascular endothelial cells (marked by alpha-SMA expression) (marked by CD31 expression). Result: There were signifi cant differences in the expression of cTnT, alpha-SMA, and CD31 (p = 0.003, p <0.001, and p <0.001, respectively) in each group of DFAT cells that received reversine. From post-hoc analysis with Tukey test, it was found that only the 10 nM reversine group produced a signifi cant difference compared to the control group (p = 0.002) for cTnT expression and reversine 10 nM and 20 nM group for -SMA expression and CD31 expression (p = 0.028 and p <0.001, respectively). Conclusions: This study proves that there is a relationship between reversine and increased plasticity of DFAT cells into cardiac derived cells in the form of cardiomyocytes (cTnT), VSMCs (alpha-SMA), and vascular endothelial cells (CD31).

Original languageEnglish
Pages (from-to)589-594
Number of pages6
JournalCor et Vasa
Volume64
Issue number6
DOIs
Publication statusPublished - Dec 2022

Keywords

  • Cardiomyocyte
  • DFAT
  • Reversine
  • VSMCs
  • Vascular endothelial cells

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