Aquaporine 4 Expression on End Feet Astrocyte Before and After Cerebrospinal Fluid Drainage of Hydrocephalus Mice Model

Background: Aquaporin (AQP) is a family of integral membrane proteins that function as water channels. AQP facilitates the transport of water across the plasma cell membrane. AQP lining the periventricular wall in the presence of edema may impair the function of the AQP to prevent or facilitate proper movement of water. Result: We analyze the effect of hydrocephalus and CSF drainage on the expression levels of aquaporin 4 (AQP4) end feet astrocytes in a hydrocephalus mice model. The test was carried out using a mice model of hydrocephalus induced with kaolin, then CSF drainage was performed on the 7th and 14th day, and compared the levels of AQP4 expression in each group. Data showed an increase in AQP4 excretion levels in astrocyte end feet along with the duration of hydrocephalus (p = 0.001) in comparison between hydrocephalus mice on the 7th, 14th, and 21st days. AQP4 before and after CSF drainage, comparison of the hydrocephalus group on day 21 with the group of mice undergoing CSF drainage (p<0.05). The results showed that the CSF drainage treatment was proven to reduce the level of AQP4. Conclusion: This is the first study to describe immunohistochemical distribution of AQP4 after drainage hydrocephalus model in mice end feet astrocyte. The AQP4 expression and distribution in after drainage hydrocephalus model was comparable 14th and 21st day of hydrocephalus but 7 days after drainage. Larger studies are needed to substantiate the influence of breed and ageing on AQP4 expression after drainage of hydrocephalus model.