TY - GEN
T1 - Application of red laser photodynamic for in vivo diabetic wound healing therapy
AU - Lailatuzzuhria, D. A.
AU - Effendi, T. A.
AU - Winarni, D.
AU - Apsari, R.
N1 - Publisher Copyright:
© 2017 IEEE.
PY - 2017/11/29
Y1 - 2017/11/29
N2 - This photodynamic research used chlorophyll photosensitizer and 634nm diode laser to enhance diabetic wound healing rate. 30 male mice (Mus musculus) were divided randomly into six groups, i.e. normal control group, diabetic control group with and without metformin, treatment group with laser beam energy density D1 (1.4 J/cm2), D2 (2.16 J/cm2), and D3 (2.85 J/cm2). An alloxan injection was used to induce diabetic condition (0.13 mg/g mice weight) via intraperitoneal injection. After the topical photosensitizer injection was done, the wounds were exposed with laser beam on the first day the incision wound has been made. Each wound length was measured every day with millimeter ruler. For the wound width, it was measured after Hematoxylin and Eosin staining. The skin tissue was sacrificed on the third day for the histology sample preparation. Laser with energy densities of 1.42 J/cm2, 2.16 J/cm2, and 2.85 J/cm2 showed positive results in the wound healing acceleration. The optimum energy density found is 1.42 J/cm2. For the next research, it is necessary to find optimum energy density around 1.42 J/cm2.
AB - This photodynamic research used chlorophyll photosensitizer and 634nm diode laser to enhance diabetic wound healing rate. 30 male mice (Mus musculus) were divided randomly into six groups, i.e. normal control group, diabetic control group with and without metformin, treatment group with laser beam energy density D1 (1.4 J/cm2), D2 (2.16 J/cm2), and D3 (2.85 J/cm2). An alloxan injection was used to induce diabetic condition (0.13 mg/g mice weight) via intraperitoneal injection. After the topical photosensitizer injection was done, the wounds were exposed with laser beam on the first day the incision wound has been made. Each wound length was measured every day with millimeter ruler. For the wound width, it was measured after Hematoxylin and Eosin staining. The skin tissue was sacrificed on the third day for the histology sample preparation. Laser with energy densities of 1.42 J/cm2, 2.16 J/cm2, and 2.85 J/cm2 showed positive results in the wound healing acceleration. The optimum energy density found is 1.42 J/cm2. For the next research, it is necessary to find optimum energy density around 1.42 J/cm2.
KW - Chlorophyll
KW - Diabetic wound
KW - Red laser
UR - http://www.scopus.com/inward/record.url?scp=85043483245&partnerID=8YFLogxK
U2 - 10.1109/ISSIMM.2017.8124267
DO - 10.1109/ISSIMM.2017.8124267
M3 - Conference contribution
AN - SCOPUS:85043483245
T3 - Proceedings - 2017 International Seminar on Sensor, Instrumentation, Measurement and Metrology: Innovation for the Advancement and Competitiveness of the Nation, ISSIMM 2017
SP - 82
EP - 85
BT - Proceedings - 2017 International Seminar on Sensor, Instrumentation, Measurement and Metrology
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2017 International Seminar on Sensor, Instrumentation, Measurement and Metrology, ISSIMM 2017
Y2 - 25 August 2017 through 26 August 2017
ER -
