Application of probiotics and microalgae (Chaetoceros calcitrans) to stimulate non-specific immune responses in white shrimp (litopenaeus vannamei) infected with vibrio harveyi

This study aims to determine whether the administration of probiotics and microalgae Chaetoceros calcitrans can improve the non-specific immune response of white shrimp after Vibrio harveyi bacterial infection. This research is experimental with a completely randomized design (CRD) consisting of 5 treatments and 4 replications.-C: Rearing shrimp without the administration of probiotics or microalgae Chaetoceros calcitrans, the 15th day was injected with PBS (Phosphate Buffer Saline). +C: Rearing shrimp without the provision of probiotics or microalgae Chaetoceros calcitrans, and on the 15th day infected with Vibrio harveyi. PA: Shrimp maintenance by providing Probiotics (Pro). PB: Shrimp maintenance by giving Microalgae (Mic). PC: Shrimp maintenance by providing Probiotics and Microalgae (Promic) for 14 days, and on 15^th day infected with V. harveyi. The parameters observed included Total Haemocyte Count (THC), Phagocytic activity. Data analysis used Analysis of Variance (ANOVA) followed by Duncan’s multiple range test (DMRT). The results of this study indicate that the administration of probiotics and microalgae gave significantly different results (p<0.05) on non-specific immune responses in white shrimp. The best research results on the treatment of probiotics and microalgae Chaetoceros calcitrans are able to increase the non-specific immune response in white shrimp after Vibrio harveyi bacterial infection THC value of 42.86 × 10⁶ cells/mL, phagocytic activity (PA) activity by 95.50%, SR 94.75%. Probiotics (Pro), Microalgae (Mic) Chaetoceros calcitrans and Combination Probiotics and Microalgae (Promic) on the maintenance media can increase non-specific immune response and survival rate in white shrimp after V. harveyi bacterial infection.