TY - GEN
T1 - Application of Fish Flour Liquid Waste as Alternative Growth Media for Producing Xylanase
AU - Budiman, Frederick
AU - Nurrohman, Ardiana Ilham
AU - Ekwanda, Refka Revina Melyata
AU - Wicaksono, Ginarto Arif
AU - Nisa, Khairun
AU - Yamani, Laura Navika
AU - Ilias, Rosli Md
AU - Puspaningsih, Ni Nyoman Tri
N1 - Publisher Copyright:
© 2023 American Institute of Physics Inc.. All rights reserved.
PY - 2023/1/4
Y1 - 2023/1/4
N2 - Consumption of processed fish product increase year by year, especially fish flour. However, processing fish flour produce untreated wastewater that contains rich element of carbon, nitrogen, and mineral. Those rich elements can be used as alternative bacterial nutrient media. This research aims to devise a combine formulation between fish flour liquid waste and Luria Bertani media. The combine medium was used to grow recombinant Eschericia coli DH5α harboring gene encoding xylanolytic enzyme of pTP510 with ratio of combine medium 1:2, 1:2, and 1:4 compared to Luria Bertani media itself. The result showed that the optimum combine formulation was 1:2 and indicated its activity of 0.7260 Unit/mL and 0.6213 Unit/mL after 9 hours and 12 hours incubation time, respectively.
AB - Consumption of processed fish product increase year by year, especially fish flour. However, processing fish flour produce untreated wastewater that contains rich element of carbon, nitrogen, and mineral. Those rich elements can be used as alternative bacterial nutrient media. This research aims to devise a combine formulation between fish flour liquid waste and Luria Bertani media. The combine medium was used to grow recombinant Eschericia coli DH5α harboring gene encoding xylanolytic enzyme of pTP510 with ratio of combine medium 1:2, 1:2, and 1:4 compared to Luria Bertani media itself. The result showed that the optimum combine formulation was 1:2 and indicated its activity of 0.7260 Unit/mL and 0.6213 Unit/mL after 9 hours and 12 hours incubation time, respectively.
UR - http://www.scopus.com/inward/record.url?scp=85146500174&partnerID=8YFLogxK
U2 - 10.1063/5.0111694
DO - 10.1063/5.0111694
M3 - Conference contribution
AN - SCOPUS:85146500174
T3 - AIP Conference Proceedings
BT - 1st International Conference on Neuroscience and Learning Technology, ICONSATIN 2021
A2 - Kristiana, Arika Indah
A2 - Alfarisi, Ridho
PB - American Institute of Physics Inc.
T2 - 1st International Conference on Neuroscience and Learning Technology, ICONSATIN 2021
Y2 - 18 September 2021 through 19 September 2021
ER -