Background: Annona squamosa is reported has a significant cytotoxic activity in some cancer cells. Objectives: Thus, this study aim to investigate Annona squamosa leaf extract induced by chitosan nanoparticles (nano-ASLE) to enhance their biological activity as anticancer agent on HeLa cells. Methods: Nano-ASLE (50, 100, 200, 400 µg/mL in DMSO) given on HeLa cells to determined IC50 value by MTT assay. Then, it was devided into three groups as follow IC50, 2IC50, 4IC50 continued with analysis of caspase-3 expression. Results: The present study demonstrated that nano-ASLE can surpress HeLa cells proliferation with the IC50 value of 344.48 µg/mL and rapid enhancement of caspase-3 activity has the mean score of 65.3 cell expression and the lowest score shows 45.3 cell expression. Conclusion: Nano-ASLE lead to HeLa cell death via the mitochondrial pathway on caspase-3 expression. In addition, the further studies are needed to obtain the loading efficiency, release of drug concentration and in vivo study of nano-ASLE to suppress HeLa cells.

Original languageEnglish
Pages (from-to)416-421
Number of pages6
JournalIndian Journal of Pharmaceutical Education and Research
Issue number2
Publication statusPublished - 1 Apr 2020


  • Annona squamosa
  • Apoptosis
  • Caspase-3
  • Cytotoxicity
  • Nanoparticles


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