Antiviral action of curcumin encapsulated in nanoemulsion against four serotypes of dengue virus

Najwa Nabila; Nadia Khansa Suada; Dionisius Denis; Benediktus Yohan; Annis Catur Adi; Anna Surgean Veterini; Atsarina Larasati Anindya; R. Tedjo Sasmono; Heni Rachmawati

doi:10.2174/2211738507666191210163408

Antiviral action of curcumin encapsulated in nanoemulsion against four serotypes of dengue virus

Najwa Nabila, Nadia Khansa Suada, Dionisius Denis, Benediktus Yohan, Annis Catur Adi, Anna Surgean Veterini, Atsarina Larasati Anindya, R. Tedjo Sasmono, Heni Rachmawati

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Background: Curcumin has been used as a traditional medicine showing anti-inflammatory, antimicrobial, and antiviral properties. Despite the promising potentials, curcumin-based drug development is hindered due to its poor solubility and cell uptake. Objective: This study aims to produce curcumin nanoemulsion (nanocurcumin) and evaluate its physical characteristics and in vitro cell cytotoxicity and antiviral activity against dengue virus (DENV). Methods: Nanocurcumin was generated by self-nanoemulsion technique. Cytotoxicity was determined using MTT assay in A549 cell line. Anti-DENV properties were determined by calculation of inhibitory concentration 50 (IC₅₀) and plaque assay. Results: The resulting nanoemulsion showed uniform droplet size distribution with the average droplet size of 40.85 ± 0.919 nm. Nanocurcumin exhibited higher cell cytotoxicity compared to curcumin solution and may be explained by better cell uptake. Nanocurcumin treatment suppressed DENV growth, although no significant difference observed compared to the curcumin solution counterpart. Greater virus reduction was observed for DENV-1 and DENV-2. Conclusion: The synthesis of nanocurcumin improved curcumin physicochemical properties with potential as antiviral against DENV.

Original language	English
Pages (from-to)	54-62
Number of pages	9
Journal	Pharmaceutical Nanotechnology
Volume	8
Issue number	1
DOIs	https://doi.org/10.2174/2211738507666191210163408
Publication status	Published - 2020

Keywords

A549 cell line
Antiviral activity
Cellular uptake
Curcumin
Dengue virus
Nanoemulsion

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2174/2211738507666191210163408

Cite this

@article{d5422e53827343d68147894e06ee6c55,

title = "Antiviral action of curcumin encapsulated in nanoemulsion against four serotypes of dengue virus",

abstract = "Background: Curcumin has been used as a traditional medicine showing anti-inflammatory, antimicrobial, and antiviral properties. Despite the promising potentials, curcumin-based drug development is hindered due to its poor solubility and cell uptake. Objective: This study aims to produce curcumin nanoemulsion (nanocurcumin) and evaluate its physical characteristics and in vitro cell cytotoxicity and antiviral activity against dengue virus (DENV). Methods: Nanocurcumin was generated by self-nanoemulsion technique. Cytotoxicity was determined using MTT assay in A549 cell line. Anti-DENV properties were determined by calculation of inhibitory concentration 50 (IC50) and plaque assay. Results: The resulting nanoemulsion showed uniform droplet size distribution with the average droplet size of 40.85 ± 0.919 nm. Nanocurcumin exhibited higher cell cytotoxicity compared to curcumin solution and may be explained by better cell uptake. Nanocurcumin treatment suppressed DENV growth, although no significant difference observed compared to the curcumin solution counterpart. Greater virus reduction was observed for DENV-1 and DENV-2. Conclusion: The synthesis of nanocurcumin improved curcumin physicochemical properties with potential as antiviral against DENV.",

keywords = "A549 cell line, Antiviral activity, Cellular uptake, Curcumin, Dengue virus, Nanoemulsion",

author = "Najwa Nabila and Suada, {Nadia Khansa} and Dionisius Denis and Benediktus Yohan and Adi, {Annis Catur} and Veterini, {Anna Surgean} and Anindya, {Atsarina Larasati} and Sasmono, {R. Tedjo} and Heni Rachmawati",

note = "Funding Information: This study was funded by the Indonesia Ministry of Research, Technology, and Higher Educa- Funding Information: This study was funded by the Indonesia Ministry of Research, Technology, and Higher Educa-tion, contract number: 127/SP2H/PTNBH/DRPM/ 2018. We thank Ms. Nur Ita Margyaningsih and La-bibah Qotrunnada, expertise in the Transmission Electron Microscopy (TEM) unit of Eijkman Institute for Molecular Biology for the TEM imaging. Publisher Copyright: {\textcopyright} 2020 Bentham Science Publishers.",

year = "2020",

doi = "10.2174/2211738507666191210163408",

language = "English",

volume = "8",

pages = "54--62",

journal = "Pharmaceutical Nanotechnology",

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T1 - Antiviral action of curcumin encapsulated in nanoemulsion against four serotypes of dengue virus

AU - Nabila, Najwa

AU - Suada, Nadia Khansa

AU - Denis, Dionisius

AU - Yohan, Benediktus

AU - Adi, Annis Catur

AU - Veterini, Anna Surgean

AU - Anindya, Atsarina Larasati

AU - Sasmono, R. Tedjo

AU - Rachmawati, Heni

N1 - Funding Information: This study was funded by the Indonesia Ministry of Research, Technology, and Higher Educa- Funding Information: This study was funded by the Indonesia Ministry of Research, Technology, and Higher Educa-tion, contract number: 127/SP2H/PTNBH/DRPM/ 2018. We thank Ms. Nur Ita Margyaningsih and La-bibah Qotrunnada, expertise in the Transmission Electron Microscopy (TEM) unit of Eijkman Institute for Molecular Biology for the TEM imaging. Publisher Copyright: © 2020 Bentham Science Publishers.

PY - 2020

Y1 - 2020

N2 - Background: Curcumin has been used as a traditional medicine showing anti-inflammatory, antimicrobial, and antiviral properties. Despite the promising potentials, curcumin-based drug development is hindered due to its poor solubility and cell uptake. Objective: This study aims to produce curcumin nanoemulsion (nanocurcumin) and evaluate its physical characteristics and in vitro cell cytotoxicity and antiviral activity against dengue virus (DENV). Methods: Nanocurcumin was generated by self-nanoemulsion technique. Cytotoxicity was determined using MTT assay in A549 cell line. Anti-DENV properties were determined by calculation of inhibitory concentration 50 (IC50) and plaque assay. Results: The resulting nanoemulsion showed uniform droplet size distribution with the average droplet size of 40.85 ± 0.919 nm. Nanocurcumin exhibited higher cell cytotoxicity compared to curcumin solution and may be explained by better cell uptake. Nanocurcumin treatment suppressed DENV growth, although no significant difference observed compared to the curcumin solution counterpart. Greater virus reduction was observed for DENV-1 and DENV-2. Conclusion: The synthesis of nanocurcumin improved curcumin physicochemical properties with potential as antiviral against DENV.

AB - Background: Curcumin has been used as a traditional medicine showing anti-inflammatory, antimicrobial, and antiviral properties. Despite the promising potentials, curcumin-based drug development is hindered due to its poor solubility and cell uptake. Objective: This study aims to produce curcumin nanoemulsion (nanocurcumin) and evaluate its physical characteristics and in vitro cell cytotoxicity and antiviral activity against dengue virus (DENV). Methods: Nanocurcumin was generated by self-nanoemulsion technique. Cytotoxicity was determined using MTT assay in A549 cell line. Anti-DENV properties were determined by calculation of inhibitory concentration 50 (IC50) and plaque assay. Results: The resulting nanoemulsion showed uniform droplet size distribution with the average droplet size of 40.85 ± 0.919 nm. Nanocurcumin exhibited higher cell cytotoxicity compared to curcumin solution and may be explained by better cell uptake. Nanocurcumin treatment suppressed DENV growth, although no significant difference observed compared to the curcumin solution counterpart. Greater virus reduction was observed for DENV-1 and DENV-2. Conclusion: The synthesis of nanocurcumin improved curcumin physicochemical properties with potential as antiviral against DENV.

KW - A549 cell line

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Antiviral action of curcumin encapsulated in nanoemulsion against four serotypes of dengue virus

Abstract

Keywords

UN SDGs

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