Antisperm antibody in repeat breeder Friesian Holstein cows at KPSP Setia Kawan Nongkojajar, Tutur district, Pasuruan, Indonesia

This study presents a laboratory explorative method by using sample of cervical mucus and blood serum from 11 Holstein Friesian (HF) cows, aged 4 to 9 years age, normal estrus cycle and gave birth before. They were divided into 2 groups, 10 dairy cows that have history of repeat breeders (4 times or more AI) (RB) and 1 normal breeder (2 times AI) as a control (C). Samples were examined using indirect-ELISA test method. The results of this study indicate that the antisperm antibody concentration that appears in cervical mucus samples and blood sera of control group has a lower concentration value (0.342 and 54.860 ng/mL) than RB group (233,776 and 944,531 ng/ml).