TY - JOUR
T1 - Antioxidant effects of graptophyllum pictum leaf extract on malondialdehyde (MDA) levels of mice induced by a toxic dose of paracetamol
AU - Kusumaningsih, Tuti
AU - Firdausi, Anita
AU - Diyatri, Indeswati
AU - Ridwan, Rini D.
AU - Arundina, Ira
AU - Yuliati,
N1 - Publisher Copyright:
© Journal of Krishna Institute of Medical Sciences University.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Background: Antioxidants are important substances which possess the ability to protect the body from damage caused by free radicals-induced oxidative stress. A shift in the balance between oxidants and antioxidants promotes oxidative stress. Graptophyllum pictum (GP) contains substances that are efficacious as antioxidants. Aim and Objectives:To prove the antioxidant effects of GP leaf extract on Malondialdehyde (MDA) levels of mice induced by a toxic dose of paracetamol. Material and Methods: Thirty mice were randomly divided into five groups (n=6); Negative Control (NC) was without treatment, Positive Control (PC) with aquadest + paracetamol, T1: GP leaf extract 150 mg/kg BW + paracetamol, T2: GP leaf extract 300 mg/kg BW+ paracetamol, T3: GP leaf extract 600 mg/kg BW+ paracetamol. Extracts were given on the 1st to 10th day and paracetamol induction was performed on the 8th, 9th, and 10th days. On the 11th day, blood serum samples were collected and the level of MDAwas then measured Results: T1, T2, T3 had the lower number of MDA levels when compared with the PC (3.625±0.374, 3.147±0.222, 2.574±0.319). One way Analysis of Variance (ANOVA) and post hoc test with Tukey showed that there was a significant difference between PC and T1, T2, T3 (p<0.05). Conclusion: GP extract has an antioxidant effect on the prevention of elevated MDA levels. A dose of 600 mg/kg BW was found to be the most effective in preventing elevation of MDA levels after a toxic dose of paracetamol was induced.
AB - Background: Antioxidants are important substances which possess the ability to protect the body from damage caused by free radicals-induced oxidative stress. A shift in the balance between oxidants and antioxidants promotes oxidative stress. Graptophyllum pictum (GP) contains substances that are efficacious as antioxidants. Aim and Objectives:To prove the antioxidant effects of GP leaf extract on Malondialdehyde (MDA) levels of mice induced by a toxic dose of paracetamol. Material and Methods: Thirty mice were randomly divided into five groups (n=6); Negative Control (NC) was without treatment, Positive Control (PC) with aquadest + paracetamol, T1: GP leaf extract 150 mg/kg BW + paracetamol, T2: GP leaf extract 300 mg/kg BW+ paracetamol, T3: GP leaf extract 600 mg/kg BW+ paracetamol. Extracts were given on the 1st to 10th day and paracetamol induction was performed on the 8th, 9th, and 10th days. On the 11th day, blood serum samples were collected and the level of MDAwas then measured Results: T1, T2, T3 had the lower number of MDA levels when compared with the PC (3.625±0.374, 3.147±0.222, 2.574±0.319). One way Analysis of Variance (ANOVA) and post hoc test with Tukey showed that there was a significant difference between PC and T1, T2, T3 (p<0.05). Conclusion: GP extract has an antioxidant effect on the prevention of elevated MDA levels. A dose of 600 mg/kg BW was found to be the most effective in preventing elevation of MDA levels after a toxic dose of paracetamol was induced.
KW - Antioxidant
KW - Free radicals
KW - Graptophyllum pictum
KW - Malondialdehyde
KW - Paracetamol
UR - http://www.scopus.com/inward/record.url?scp=85051061021&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85051061021
SN - 2231-4261
VL - 7
SP - 59
EP - 64
JO - Journal of Krishna Institute of Medical Sciences University
JF - Journal of Krishna Institute of Medical Sciences University
IS - 3
ER -