Antioxidant, anti-inflammatory and anti-acne activities of stingless bee (Tetragonula biroi) propolis

Enos Tangke Arung, Syafrizal, Irawan Wijaya Kusuma, Swandari Paramita, Yhiya Amen, Yong Ung Kim, Netty Maria Naibaho, Rico Ramadhan, Harits Atika Ariyanta, Widya Fatriasari, Kuniyoshi Shimizu

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


We collected stingless bee propolis Tetragonula biroi in order to find materials for medicine and cosmetics applications from tropical rainforest resources. Even though this bee has some biological functions including a cancer cell line, hair growth promotion, asthma remedy, α-glucosidase enzyme inhibition, and antiviral action, the investigation on anti-acne has not been reported yet. This study was to focus on propolis Tetragonula biroi extracts and leads us to isolate active compounds for antioxidant, anti-inflammatory, and anti-acne. We used methanol to obtain the extract from this propolis and assayed it with antioxidants, anti-inflammation, and anti-acne. The extract showed strong activity in antioxidants by DPPH radical scavenging activity (82.31% in 6.25 μg/ml). Via a column chromatography and Reveleris PREP purification system, we isolated 3’–O–methyldiplacone, nymphaeol A, and 5,7,3′,4′–tetrahydroxy–6–geranyl flavonol. These compounds showed potential biological activity with IC50 for antioxidant 6.33, 15.49, 17.32 μM; and antiinflammatory 121.54, 121.20, 117.31 μM. The isolated compounds showed anti-acne properties with properties 0.00, 14.11, and 13.78 mm for the inhibition zone (at a concentration of 1 μg/well), respectively. The results indicated that the propolis extract of Tetragonula biroi has the potential to be developed as a cosmetic agent; however, further work needs to be done to clarify its application.

Original languageEnglish
Article number105375
Publication statusPublished - Jan 2023


  • Anti-acne
  • Anti-inflammatory
  • Antioxidant
  • Tetragonula biroi
  • propolis


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