Antimicrobial resistance patterns and genes of Campylobacter jejuni isolated from chickens in Pasuruan, Indonesia

Sheila Marty Yanestria, Mustofa Helmi Effendi, Wiwiek Tyasningsih, Ikechukwu Benjamin Moses, Aswin Rafif Khairullah, Shendy Canadya Kurniawan, Fidi Nur Aini Eka Puji Dameanti, Rosmita Ikaratri, Junianto Wika Adi Pratama, Miarsono Sigit, Abdullah Hasib, Otto Sahat Martua Silaen

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Poultry is one of the most prominent sources of Campylobacter jejuni, which is also a major means of transmission to people. Campylobacter jejuni contamination in chicken meat comes from chicken feces because it naturally exists in the intestines of chickens. Aim: The purpose of this study is to identify the antibiotic resistance patterns and genes of C. jejuni, which was found in chickens in Pasuruan, Indonesia. Methods: The samples used in this study were 200 contents of the small intestine of broiler chickens from 40 farms in Pasuruan Regency. The enriched sample was streaked on the selective media of modified charcoal cefoperazone deoxycholate agar containing the CCDA selective supplement. Antimicrobial susceptibility test utilizing the Kirby– Bauer diffusion test method in accordance with Clinical and Laboratory Standards Institute standards. The polymerase chain reaction (PCR) method was used to detect the (hipO), which encodes the C. jejuni strain, fluoroquinolone resistance (gyrA), beta-lactam resistance (blaOXA-61), and tetracycline resistance (tetO) genes. Results: The findings revealed a 14% (28/200) prevalence of C. jejuni in the small intestine of broiler chickens. These isolates showed high resistance to enrofloxacin (92.9%). All isolates (100%) were susceptible to amoxicillin-clavulanate. The PCR results showed all C. jejuni isolates (100%) detected the gyrA gene, 96.4% detected the blaOXA-61 gene, and 50% detected the tetO gene. Conclusion: The findings of antimicrobial resistance at a high level from the small intestine of broiler chickens illustrate the potential threat to human health. To lessen the effects now and in the future, coordinated and suitable action is needed, as well as steps to guarantee the poultry industry’s economic survival and public health insurance.

Original languageEnglish
Pages (from-to)759-768
Number of pages10
JournalOpen Veterinary Journal
Volume14
Issue number3
DOIs
Publication statusPublished - 2024

Keywords

  • Antimicrobial resistance
  • Campylobacter jejuni
  • Chickens
  • Gene
  • Indonesia

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