TY - JOUR
T1 - Antimicrobial activity of actinomycetes isolated from mangrove soil in Tuban, Indonesia
AU - Fatimah,
AU - Suroiyah, Fatichatus
AU - Solikha, Nikmatus
AU - Rahayuningtyas, Nazil Dwi
AU - Surtiningsih, Tini
AU - Nurhariyati, Tri
AU - Ni’matuzahroh, null
AU - Affandi, Moch
AU - Geraldi, Almando
AU - Thontowi, Ahmad
N1 - Funding Information:
The authors would like to thank Universitas Airlangga, Surabaya, Indonesia for funding the research through the Penelitian Unggulan Fakultas (PUF) 2021 scheme with contract number 2727/UN3.1.8 /PT/2021.
Publisher Copyright:
© 2022, Society for Indonesian Biodiversity. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Antimicrobial activity of actinomycetes isolated from mangrove soil in Tuban, Indonesia. Biodiversitas 23: 2957-2965. This study aims to isolate, identify, and investigate the antimicrobial activity of actinomycetes isolated from mangrove soil of the Mangrove Center Tuban in Jenu Village, Tuban District, Indonesia. Soil sampling was carried out with a purposive sampling technique. The dry-heating and pour plate methods were used to isolate actinomycetes. Four isolates (LIB.2; LIA.4; LIA.9; and LIA.10) were selected and tested for their antimicrobial activities against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Candida albicans ATCC 10231 using disc diffusion method. Colony morphology characterization and Gram staining were then conducted on the 4 isolates. The identification of LIB.2 was performed by biochemical assay and 16S rRNA gene sequence analysis. The results showed that crude antimicrobial extracts (CACs) of all isolates inhibited the growth of E. coli but not S. aureus and C. albicans. Isolate LIB.2 showed the highest inhibition zone with a diameter of 15,65 ± 2,33 mm, followed by LIA.9 (10,19 ± 2,78 mm), LIA.4 (9,80 ± 0,47), and LIA.10 (8,75 ± 0,42 mm). The most potential isolate was identified as a member of Streptomyces genera. Based on biochemical assay and 16S rRNA gene sequence analysis, LIB.2 was identified as Streptomyces vellosus strain LIB.2.
AB - Antimicrobial activity of actinomycetes isolated from mangrove soil in Tuban, Indonesia. Biodiversitas 23: 2957-2965. This study aims to isolate, identify, and investigate the antimicrobial activity of actinomycetes isolated from mangrove soil of the Mangrove Center Tuban in Jenu Village, Tuban District, Indonesia. Soil sampling was carried out with a purposive sampling technique. The dry-heating and pour plate methods were used to isolate actinomycetes. Four isolates (LIB.2; LIA.4; LIA.9; and LIA.10) were selected and tested for their antimicrobial activities against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Candida albicans ATCC 10231 using disc diffusion method. Colony morphology characterization and Gram staining were then conducted on the 4 isolates. The identification of LIB.2 was performed by biochemical assay and 16S rRNA gene sequence analysis. The results showed that crude antimicrobial extracts (CACs) of all isolates inhibited the growth of E. coli but not S. aureus and C. albicans. Isolate LIB.2 showed the highest inhibition zone with a diameter of 15,65 ± 2,33 mm, followed by LIA.9 (10,19 ± 2,78 mm), LIA.4 (9,80 ± 0,47), and LIA.10 (8,75 ± 0,42 mm). The most potential isolate was identified as a member of Streptomyces genera. Based on biochemical assay and 16S rRNA gene sequence analysis, LIB.2 was identified as Streptomyces vellosus strain LIB.2.
KW - Actinomycetes
KW - Mangrove Center Tuban
KW - Streptomyces vellosus strain LIB.2
KW - antimicrobial compounds
KW - human welfare
KW - microbial diversity
UR - http://www.scopus.com/inward/record.url?scp=85132249988&partnerID=8YFLogxK
U2 - 10.13057/biodiv/d230622
DO - 10.13057/biodiv/d230622
M3 - Article
AN - SCOPUS:85132249988
SN - 1412-033X
VL - 23
SP - 2957
EP - 2965
JO - Biodiversitas
JF - Biodiversitas
IS - 6
ER -