Antihepatitis C virus activity of indonesian mahogany (Toona Sureni)

Achmad Fuad Hafid; Tutik Sri Wahyuni; Lidya Tumewu; Evhy Apryani; Adita Ayu Permanasari; Myrna Adianti; Chie Aoki Utsubo; Aty Widyawaruyanti; Maria Inge Lusida; Hiroyuki Fuchino; Nobuo Kawahara; Hak Hotta

doi:10.22159/ajpcr.2018.v11i2.18126

Antihepatitis C virus activity of indonesian mahogany (Toona Sureni)

Achmad Fuad Hafid, Tutik Sri Wahyuni, Lidya Tumewu, Evhy Apryani, Adita Ayu Permanasari, Myrna Adianti, Chie Aoki Utsubo, Aty Widyawaruyanti, Maria Inge Lusida, Soetjipto, Hiroyuki Fuchino, Nobuo Kawahara, Hak Hotta

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Objective: Toona sureni (Indonesian mahogany) is a member of Meliaceae family and locally known as suren. Previous study reported that T. sureni leaves extract exhibited antiviral activity with 50% inhibitory concentration (IC₅₀) value of 13.9 ± 1.6 µg/ml against hepatitis C virus (HCV) J6/JFH1. Cytotoxicity analysis of T. sureni leaves extract did not reveal any cytotoxicity effect; therefore, further study was taken to investigate the active substances from the extract. Methods: Bioassay-guided isolation of anti-HCV was conducted using Huh-7.5 cells infected with HCV J6/JFH1 in the presence of extracts, fractions, or compounds from the plant. Results: Ethyl acetate fraction (Fr E) exhibited high anti-HCV activity with IC₅₀ value of 1.7 µg/ml. Further, separation of Fr E by open column chromatography resulted in nine sub-fractions (sub-Fr E1-E9). Sub-Fr E3 and E4 have IC₅₀ value of 29.90 µg/ml and 7.68 µg/ml, respectively. Polyphenols compounds have been isolated from sub-Fr E3 and E4. The structures have been determined to be ethyl gallate (1), methyl gallate (2), catechin (3), gallic acid (4), and quercetin 3-O-rhamnoside (5). Among the isolated compounds, gallic acid showed to possess strong anti-HCV activity with IC₅₀ value of 15.9 µg/ml. Conclusion: T. sureni and its isolated compound, gallic acid, may be good candidates to develop for alternative and/or complementary agents of anti-HCV infection.

Original language	English
Pages (from-to)	87-90
Number of pages	4
Journal	Asian Journal of Pharmaceutical and Clinical Research
Volume	11
Issue number	2
DOIs	https://doi.org/10.22159/ajpcr.2018.v11i2.18126
Publication status	Published - Feb 2018

Keywords

Antihepatitis C virus
Gallic acid
Leaves extract
Polyphenols
Toona sureni

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.22159/ajpcr.2018.v11i2.18126

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Hafid, A. F., Wahyuni, T. S., Tumewu, L., Apryani, E., Permanasari, A. A., Adianti, M., Utsubo, C. A., Widyawaruyanti, A., Lusida, M. I., Soetjipto, Fuchino, H., Kawahara, N., & Hotta, H. (2018). Antihepatitis C virus activity of indonesian mahogany (Toona Sureni). Asian Journal of Pharmaceutical and Clinical Research, 11(2), 87-90. https://doi.org/10.22159/ajpcr.2018.v11i2.18126

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title = "Antihepatitis C virus activity of indonesian mahogany (Toona Sureni)",

abstract = "Objective: Toona sureni (Indonesian mahogany) is a member of Meliaceae family and locally known as suren. Previous study reported that T. sureni leaves extract exhibited antiviral activity with 50% inhibitory concentration (IC50) value of 13.9 ± 1.6 µg/ml against hepatitis C virus (HCV) J6/JFH1. Cytotoxicity analysis of T. sureni leaves extract did not reveal any cytotoxicity effect; therefore, further study was taken to investigate the active substances from the extract. Methods: Bioassay-guided isolation of anti-HCV was conducted using Huh-7.5 cells infected with HCV J6/JFH1 in the presence of extracts, fractions, or compounds from the plant. Results: Ethyl acetate fraction (Fr E) exhibited high anti-HCV activity with IC50 value of 1.7 µg/ml. Further, separation of Fr E by open column chromatography resulted in nine sub-fractions (sub-Fr E1-E9). Sub-Fr E3 and E4 have IC50 value of 29.90 µg/ml and 7.68 µg/ml, respectively. Polyphenols compounds have been isolated from sub-Fr E3 and E4. The structures have been determined to be ethyl gallate (1), methyl gallate (2), catechin (3), gallic acid (4), and quercetin 3-O-rhamnoside (5). Among the isolated compounds, gallic acid showed to possess strong anti-HCV activity with IC50 value of 15.9 µg/ml. Conclusion: T. sureni and its isolated compound, gallic acid, may be good candidates to develop for alternative and/or complementary agents of anti-HCV infection.",

keywords = "Antihepatitis C virus, Gallic acid, Leaves extract, Polyphenols, Toona sureni",

author = "Hafid, {Achmad Fuad} and Wahyuni, {Tutik Sri} and Lidya Tumewu and Evhy Apryani and Permanasari, {Adita Ayu} and Myrna Adianti and Utsubo, {Chie Aoki} and Aty Widyawaruyanti and Lusida, {Maria Inge} and Soetjipto and Hiroyuki Fuchino and Nobuo Kawahara and Hak Hotta",

note = "Publisher Copyright: {\textcopyright} 2018 The Authors.",

year = "2018",

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doi = "10.22159/ajpcr.2018.v11i2.18126",

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Hafid, AF , Wahyuni, TS, Tumewu, L, Apryani, E, Permanasari, AA, Adianti, M, Utsubo, CA, Widyawaruyanti, A , Lusida, MI , Soetjipto, Fuchino, H, Kawahara, N & Hotta, H 2018, 'Antihepatitis C virus activity of indonesian mahogany (Toona Sureni)', Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 2, pp. 87-90. https://doi.org/10.22159/ajpcr.2018.v11i2.18126

TY - JOUR

T1 - Antihepatitis C virus activity of indonesian mahogany (Toona Sureni)

AU - Hafid, Achmad Fuad

AU - Wahyuni, Tutik Sri

AU - Tumewu, Lidya

AU - Apryani, Evhy

AU - Permanasari, Adita Ayu

AU - Adianti, Myrna

AU - Utsubo, Chie Aoki

AU - Widyawaruyanti, Aty

AU - Lusida, Maria Inge

AU - Soetjipto,

AU - Fuchino, Hiroyuki

AU - Kawahara, Nobuo

AU - Hotta, Hak

PY - 2018/2

Y1 - 2018/2

N2 - Objective: Toona sureni (Indonesian mahogany) is a member of Meliaceae family and locally known as suren. Previous study reported that T. sureni leaves extract exhibited antiviral activity with 50% inhibitory concentration (IC50) value of 13.9 ± 1.6 µg/ml against hepatitis C virus (HCV) J6/JFH1. Cytotoxicity analysis of T. sureni leaves extract did not reveal any cytotoxicity effect; therefore, further study was taken to investigate the active substances from the extract. Methods: Bioassay-guided isolation of anti-HCV was conducted using Huh-7.5 cells infected with HCV J6/JFH1 in the presence of extracts, fractions, or compounds from the plant. Results: Ethyl acetate fraction (Fr E) exhibited high anti-HCV activity with IC50 value of 1.7 µg/ml. Further, separation of Fr E by open column chromatography resulted in nine sub-fractions (sub-Fr E1-E9). Sub-Fr E3 and E4 have IC50 value of 29.90 µg/ml and 7.68 µg/ml, respectively. Polyphenols compounds have been isolated from sub-Fr E3 and E4. The structures have been determined to be ethyl gallate (1), methyl gallate (2), catechin (3), gallic acid (4), and quercetin 3-O-rhamnoside (5). Among the isolated compounds, gallic acid showed to possess strong anti-HCV activity with IC50 value of 15.9 µg/ml. Conclusion: T. sureni and its isolated compound, gallic acid, may be good candidates to develop for alternative and/or complementary agents of anti-HCV infection.

AB - Objective: Toona sureni (Indonesian mahogany) is a member of Meliaceae family and locally known as suren. Previous study reported that T. sureni leaves extract exhibited antiviral activity with 50% inhibitory concentration (IC50) value of 13.9 ± 1.6 µg/ml against hepatitis C virus (HCV) J6/JFH1. Cytotoxicity analysis of T. sureni leaves extract did not reveal any cytotoxicity effect; therefore, further study was taken to investigate the active substances from the extract. Methods: Bioassay-guided isolation of anti-HCV was conducted using Huh-7.5 cells infected with HCV J6/JFH1 in the presence of extracts, fractions, or compounds from the plant. Results: Ethyl acetate fraction (Fr E) exhibited high anti-HCV activity with IC50 value of 1.7 µg/ml. Further, separation of Fr E by open column chromatography resulted in nine sub-fractions (sub-Fr E1-E9). Sub-Fr E3 and E4 have IC50 value of 29.90 µg/ml and 7.68 µg/ml, respectively. Polyphenols compounds have been isolated from sub-Fr E3 and E4. The structures have been determined to be ethyl gallate (1), methyl gallate (2), catechin (3), gallic acid (4), and quercetin 3-O-rhamnoside (5). Among the isolated compounds, gallic acid showed to possess strong anti-HCV activity with IC50 value of 15.9 µg/ml. Conclusion: T. sureni and its isolated compound, gallic acid, may be good candidates to develop for alternative and/or complementary agents of anti-HCV infection.

KW - Antihepatitis C virus

KW - Gallic acid

KW - Leaves extract

KW - Polyphenols

KW - Toona sureni

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U2 - 10.22159/ajpcr.2018.v11i2.18126

DO - 10.22159/ajpcr.2018.v11i2.18126

M3 - Article

AN - SCOPUS:85041671729

SN - 0974-2441

VL - 11

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JO - Asian Journal of Pharmaceutical and Clinical Research

JF - Asian Journal of Pharmaceutical and Clinical Research

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Antihepatitis C virus activity of indonesian mahogany (Toona Sureni)

Abstract

Keywords

UN SDGs

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