Antigenic protein profile of Anisakis spp. (Secernentea: Ascaridia: Anisakidae) larvae isolated from mackerel tuna fish (Euthynnus affinis) (Actinopterygii: Scombriformes: Scombridae)

Zafitri Nuryati Wastomi; Nunuk Dyah Retno Lastuti; Rahayu Ernawati; Lucia Tri Suwanti; Setiawan Koesdarto; Mufasirin Mufasirin; Hartanto Mulyo Raharjo

Antigenic protein profile of Anisakis spp. (Secernentea: Ascaridia: Anisakidae) larvae isolated from mackerel tuna fish (Euthynnus affinis) (Actinopterygii: Scombriformes: Scombridae)

Zafitri Nuryati Wastomi, Nunuk Dyah Retno Lastuti, Rahayu Ernawati, Lucia Tri Suwanti, Setiawan Koesdarto, Mufasirin Mufasirin, Hartanto Mulyo Raharjo

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The study aimed to explore the antigenic proteins in third-stage Anisakis spp. larvae from mackerel tuna. This research used 300 Anisakis spp. larvae from 110 mackerel tuna fish from Brondong, Lamongan, East Java, Indonesia. The research was carried out in several stages: isolation and identification of Anisakis spp.; isolation of soluble proteins and measurement by spectrophotometry; whole-protein characterization with SDS-PAGE; rabbit immunization for polyclonal antibody production; antibody titer measurement with indirect ELISA; and antigenic protein characterization via Western blot. Spectrophotometry results of the larvae showed homogenate protein level of 6833.3 μg/ml. Protein analysis of whole worm extract of the larvae using SDS-PAGE yielded 21 bands, with molecular weights between 12 kDa-238 kDa. Further, protein characterization via Western blot produced six antigenic protein bands: 64 kDa, 45 kDa, 38 kDa, 30 kDa, 27 kDa and 23 kDa. In conclusion, Anisakis spp. larvae in mackerel tuna contain six types of antigenic proteins, which might play a role in hypersensitivity or allergic reactions.

Original language	English
Pages (from-to)	85-90
Number of pages	6
Journal	Philippine Journal of Veterinary Medicine
Volume	55
Issue number	Special Issue
Publication status	Published - 1 Dec 2018
Externally published	Yes

Keywords

Anisakis spp.
Antigenic protein
Euthynnus sp.
Third-stage larvae

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Wastomi, Z. N., Lastuti, N. D. R., Ernawati, R., Suwanti, L. T., Koesdarto, S., Mufasirin, M., & Raharjo, H. M. (2018). Antigenic protein profile of Anisakis spp. (Secernentea: Ascaridia: Anisakidae) larvae isolated from mackerel tuna fish (Euthynnus affinis) (Actinopterygii: Scombriformes: Scombridae). Philippine Journal of Veterinary Medicine, 55(Special Issue), 85-90.

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title = "Antigenic protein profile of Anisakis spp. (Secernentea: Ascaridia: Anisakidae) larvae isolated from mackerel tuna fish (Euthynnus affinis) (Actinopterygii: Scombriformes: Scombridae)",

abstract = "The study aimed to explore the antigenic proteins in third-stage Anisakis spp. larvae from mackerel tuna. This research used 300 Anisakis spp. larvae from 110 mackerel tuna fish from Brondong, Lamongan, East Java, Indonesia. The research was carried out in several stages: isolation and identification of Anisakis spp.; isolation of soluble proteins and measurement by spectrophotometry; whole-protein characterization with SDS-PAGE; rabbit immunization for polyclonal antibody production; antibody titer measurement with indirect ELISA; and antigenic protein characterization via Western blot. Spectrophotometry results of the larvae showed homogenate protein level of 6833.3 μg/ml. Protein analysis of whole worm extract of the larvae using SDS-PAGE yielded 21 bands, with molecular weights between 12 kDa-238 kDa. Further, protein characterization via Western blot produced six antigenic protein bands: 64 kDa, 45 kDa, 38 kDa, 30 kDa, 27 kDa and 23 kDa. In conclusion, Anisakis spp. larvae in mackerel tuna contain six types of antigenic proteins, which might play a role in hypersensitivity or allergic reactions.",

keywords = "Anisakis spp., Antigenic protein, Euthynnus sp., Third-stage larvae",

author = "Wastomi, {Zafitri Nuryati} and Lastuti, {Nunuk Dyah Retno} and Rahayu Ernawati and Suwanti, {Lucia Tri} and Setiawan Koesdarto and Mufasirin Mufasirin and Raharjo, {Hartanto Mulyo}",

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day = "1",

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volume = "55",

pages = "85--90",

journal = "Philippine Journal of Veterinary Medicine",

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publisher = "University of the Philippines Los Banos",

number = "Special Issue",

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Antigenic protein profile of Anisakis spp. (Secernentea: Ascaridia: Anisakidae) larvae isolated from mackerel tuna fish (Euthynnus affinis) (Actinopterygii: Scombriformes: Scombridae). / Wastomi, Zafitri Nuryati; Lastuti, Nunuk Dyah Retno ; Ernawati, Rahayu et al.
In: Philippine Journal of Veterinary Medicine, Vol. 55, No. Special Issue, 01.12.2018, p. 85-90.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Antigenic protein profile of Anisakis spp. (Secernentea: Ascaridia: Anisakidae) larvae isolated from mackerel tuna fish (Euthynnus affinis) (Actinopterygii: Scombriformes: Scombridae)

AU - Wastomi, Zafitri Nuryati

AU - Lastuti, Nunuk Dyah Retno

AU - Ernawati, Rahayu

AU - Suwanti, Lucia Tri

AU - Koesdarto, Setiawan

AU - Mufasirin, Mufasirin

AU - Raharjo, Hartanto Mulyo

PY - 2018/12/1

Y1 - 2018/12/1

N2 - The study aimed to explore the antigenic proteins in third-stage Anisakis spp. larvae from mackerel tuna. This research used 300 Anisakis spp. larvae from 110 mackerel tuna fish from Brondong, Lamongan, East Java, Indonesia. The research was carried out in several stages: isolation and identification of Anisakis spp.; isolation of soluble proteins and measurement by spectrophotometry; whole-protein characterization with SDS-PAGE; rabbit immunization for polyclonal antibody production; antibody titer measurement with indirect ELISA; and antigenic protein characterization via Western blot. Spectrophotometry results of the larvae showed homogenate protein level of 6833.3 μg/ml. Protein analysis of whole worm extract of the larvae using SDS-PAGE yielded 21 bands, with molecular weights between 12 kDa-238 kDa. Further, protein characterization via Western blot produced six antigenic protein bands: 64 kDa, 45 kDa, 38 kDa, 30 kDa, 27 kDa and 23 kDa. In conclusion, Anisakis spp. larvae in mackerel tuna contain six types of antigenic proteins, which might play a role in hypersensitivity or allergic reactions.

AB - The study aimed to explore the antigenic proteins in third-stage Anisakis spp. larvae from mackerel tuna. This research used 300 Anisakis spp. larvae from 110 mackerel tuna fish from Brondong, Lamongan, East Java, Indonesia. The research was carried out in several stages: isolation and identification of Anisakis spp.; isolation of soluble proteins and measurement by spectrophotometry; whole-protein characterization with SDS-PAGE; rabbit immunization for polyclonal antibody production; antibody titer measurement with indirect ELISA; and antigenic protein characterization via Western blot. Spectrophotometry results of the larvae showed homogenate protein level of 6833.3 μg/ml. Protein analysis of whole worm extract of the larvae using SDS-PAGE yielded 21 bands, with molecular weights between 12 kDa-238 kDa. Further, protein characterization via Western blot produced six antigenic protein bands: 64 kDa, 45 kDa, 38 kDa, 30 kDa, 27 kDa and 23 kDa. In conclusion, Anisakis spp. larvae in mackerel tuna contain six types of antigenic proteins, which might play a role in hypersensitivity or allergic reactions.

KW - Anisakis spp.

KW - Antigenic protein

KW - Euthynnus sp.

KW - Third-stage larvae

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SN - 0031-7705

VL - 55

SP - 85

EP - 90

JO - Philippine Journal of Veterinary Medicine

JF - Philippine Journal of Veterinary Medicine

IS - Special Issue

ER -

Antigenic protein profile of Anisakis spp. (Secernentea: Ascaridia: Anisakidae) larvae isolated from mackerel tuna fish (Euthynnus affinis) (Actinopterygii: Scombriformes: Scombridae)

Abstract

Keywords

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