TY - JOUR
T1 - Antifungal activity of freshly squeezed garlic as denture cleanser on Candida albicans growth
AU - Nirwana, Intan
AU - Agustantina, Titien Hary
AU - Asymal, Alhidayati
N1 - Publisher Copyright:
© 2018 University of Dicle.
PY - 2018
Y1 - 2018
N2 - The objective of this study was to investigate the growth of Candida albicans on acrylic resin plate after soaking in freshly squeezed garlic. Samples were immersed in sterile saliva for 1 hour at room temperature for the formation of pellicle. They were taken and rinsed with phosphate buffer saline (PBS) solution, contaminated with Candida albicans by insertion into a tube containing Candida albicans suspension, incubated for 24 hours at 370° C, removed and rinsed with PBS solution, soaked in freshly squeezed garlic in test tubes for 3 minutes, containing 10 ml of freshly squeezed garlic of 20%, 30 %, 40 % concentration each and sterile distilled water. Samples were taken and rinsed with PBS, inserted into Sabouraud's broth, vibrated with vortex for 30 seconds to release Candida albicans attached to them. Suspension from Sabouraud's broth was taken, dripped on Sabouraud's dextrose agar, spreading, incubated for 48 hours, at 370C. Candida albicans colonies were calculated. Data were analysed using Anova and followed by LSD test. The Results showed that there were significant difference among the groups (p < 0.05). In conclusion, the higher concentration of freshly squeezed garlic the lower the number of Candida albicans colonies.
AB - The objective of this study was to investigate the growth of Candida albicans on acrylic resin plate after soaking in freshly squeezed garlic. Samples were immersed in sterile saliva for 1 hour at room temperature for the formation of pellicle. They were taken and rinsed with phosphate buffer saline (PBS) solution, contaminated with Candida albicans by insertion into a tube containing Candida albicans suspension, incubated for 24 hours at 370° C, removed and rinsed with PBS solution, soaked in freshly squeezed garlic in test tubes for 3 minutes, containing 10 ml of freshly squeezed garlic of 20%, 30 %, 40 % concentration each and sterile distilled water. Samples were taken and rinsed with PBS, inserted into Sabouraud's broth, vibrated with vortex for 30 seconds to release Candida albicans attached to them. Suspension from Sabouraud's broth was taken, dripped on Sabouraud's dextrose agar, spreading, incubated for 48 hours, at 370C. Candida albicans colonies were calculated. Data were analysed using Anova and followed by LSD test. The Results showed that there were significant difference among the groups (p < 0.05). In conclusion, the higher concentration of freshly squeezed garlic the lower the number of Candida albicans colonies.
KW - Acrylic resin
KW - Antifungal
KW - Candida albicans
KW - Freshly squeezed garlic
UR - http://www.scopus.com/inward/record.url?scp=85067046876&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85067046876
SN - 1309-100X
VL - 11
SP - 639
EP - 642
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 2
ER -