Anticancer Effect of Naringin on Human Colon Cancer (WiDr Cells): In Vitro Study

Amaq Fadholly; Arif N.M. Ansori; Budi Utomo

doi:10.52711/0974-360X.2022.00148

Anticancer Effect of Naringin on Human Colon Cancer (WiDr Cells): In Vitro Study

Amaq Fadholly, Arif N.M. Ansori, Budi Utomo

Faculty of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Naringin, a bioflavonoid naturally found in citrus species, has been exert as promising anticancer activities. However, naringin mechanism as anticancer still remain enigmatic. This study, WiDr cell lines as human colon cancer cell in vitro were used to evaluate anticancer effect of naringin. Naringin (10, 20, 40, 60, 80µg/mL) treated on WiDr cells to evaluate IC₅₀ value by MTT assay, then continued with analyzed caspase-3 expressions (IC₅₀, 2IC₅₀) as apopotosis marker. This study reported that naringin inhibit the growth of WiDr cell with IC₅₀ value is 63.14µg/mL. The caspase-3 expressions on 2IC₅₀ is higher (74.66%) than IC₅₀ (56.66%). These results indicate that naringin has properties of inhibting cell growth and inducing caspase-3 expressions on WiDr cells. It is a potential compound to be developed as a chemopreventive agent for colon cancer.

Original language	English
Pages (from-to)	885-888
Number of pages	4
Journal	Research Journal of Pharmacy and Technology
Volume	15
Issue number	2
DOIs	https://doi.org/10.52711/0974-360X.2022.00148
Publication status	Published - Feb 2022

Keywords

Apoptosis
Caspase-3
Colon cancer
Naringin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.52711/0974-360X.2022.00148

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title = "Anticancer Effect of Naringin on Human Colon Cancer (WiDr Cells): In Vitro Study",

abstract = "Naringin, a bioflavonoid naturally found in citrus species, has been exert as promising anticancer activities. However, naringin mechanism as anticancer still remain enigmatic. This study, WiDr cell lines as human colon cancer cell in vitro were used to evaluate anticancer effect of naringin. Naringin (10, 20, 40, 60, 80µg/mL) treated on WiDr cells to evaluate IC50 value by MTT assay, then continued with analyzed caspase-3 expressions (IC50, 2IC50) as apopotosis marker. This study reported that naringin inhibit the growth of WiDr cell with IC50 value is 63.14µg/mL. The caspase-3 expressions on 2IC50 is higher (74.66%) than IC50 (56.66%). These results indicate that naringin has properties of inhibting cell growth and inducing caspase-3 expressions on WiDr cells. It is a potential compound to be developed as a chemopreventive agent for colon cancer.",

keywords = "Apoptosis, Caspase-3, Colon cancer, Naringin",

author = "Amaq Fadholly and Ansori, {Arif N.M.} and Budi Utomo",

note = "Funding Information: This study was supported by PMDSU grant with the reference number 1341/UN3.14/LT/2018 from the Ministry of Research Technology and Higher Education (KEMENRISTEKDIKTI) of the Republic of Indonesia. Publisher Copyright: {\textcopyright} RJPT All right reserved.",

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doi = "10.52711/0974-360X.2022.00148",

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AU - Fadholly, Amaq

AU - Ansori, Arif N.M.

AU - Utomo, Budi

N1 - Funding Information: This study was supported by PMDSU grant with the reference number 1341/UN3.14/LT/2018 from the Ministry of Research Technology and Higher Education (KEMENRISTEKDIKTI) of the Republic of Indonesia. Publisher Copyright: © RJPT All right reserved.

PY - 2022/2

Y1 - 2022/2

N2 - Naringin, a bioflavonoid naturally found in citrus species, has been exert as promising anticancer activities. However, naringin mechanism as anticancer still remain enigmatic. This study, WiDr cell lines as human colon cancer cell in vitro were used to evaluate anticancer effect of naringin. Naringin (10, 20, 40, 60, 80µg/mL) treated on WiDr cells to evaluate IC50 value by MTT assay, then continued with analyzed caspase-3 expressions (IC50, 2IC50) as apopotosis marker. This study reported that naringin inhibit the growth of WiDr cell with IC50 value is 63.14µg/mL. The caspase-3 expressions on 2IC50 is higher (74.66%) than IC50 (56.66%). These results indicate that naringin has properties of inhibting cell growth and inducing caspase-3 expressions on WiDr cells. It is a potential compound to be developed as a chemopreventive agent for colon cancer.

AB - Naringin, a bioflavonoid naturally found in citrus species, has been exert as promising anticancer activities. However, naringin mechanism as anticancer still remain enigmatic. This study, WiDr cell lines as human colon cancer cell in vitro were used to evaluate anticancer effect of naringin. Naringin (10, 20, 40, 60, 80µg/mL) treated on WiDr cells to evaluate IC50 value by MTT assay, then continued with analyzed caspase-3 expressions (IC50, 2IC50) as apopotosis marker. This study reported that naringin inhibit the growth of WiDr cell with IC50 value is 63.14µg/mL. The caspase-3 expressions on 2IC50 is higher (74.66%) than IC50 (56.66%). These results indicate that naringin has properties of inhibting cell growth and inducing caspase-3 expressions on WiDr cells. It is a potential compound to be developed as a chemopreventive agent for colon cancer.

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KW - Caspase-3

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Anticancer Effect of Naringin on Human Colon Cancer (WiDr Cells): In Vitro Study

Abstract

Keywords

UN SDGs

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