TY - JOUR
T1 - Anticancer activity of okra raw polysaccharides extracts against human liver cancer cells
AU - Hayaza, Suhailah
AU - Wahyuningsih, Sri Puji Astuti
AU - Susilo, Raden Joko Kuncoroningrat
AU - Permanasari, Adita Ayu
AU - Husen, Saikhu Akhmad
AU - Winarni, Dwi
AU - Punnapayak, Hunsa
AU - Darmanto, Win
N1 - Publisher Copyright:
© 2019 The authors. This work is licensed under the Creative Commons Trop JAttribution Pharm Res, 4.0August International 2019; License 18(8): 1667
PY - 2019
Y1 - 2019
N2 - Purpose: To examine raw polysaccharides extract of okra (Abelmoschus esculentus L.) as an anticancer agent against human liver cancer cells. Methods: Okra raw polysaccharide extract (ORPE) was obtained by ethanol extraction from the raw fruit. Huh7it cells were grown in DMEM (Dulbecco's Modified Eagle Medium) and cultured for 24 h prior to treatment with the extract. The cell culture was divided into 3 groups, viz, negative control group (KN), positive control group (KP, treated with 10 μg/mL doxorubicin), and ORPE (P) group. ORPE group was divided into 5 subgroups based on the dose used for treatment, viz, 50 (P1), 100 (P2), 200 (P3), 400 (P4), and 600 µg/mL (P5). Huh7it cell proliferation was measured by MTT assay. while measurement of Huh7it cell apoptosis, necrosis, and cell cycle analysis were carried out using Annexin V FITC-PI antibody test and flow cytometry. Results: ORPE significantly inhibited Huh7it cell proliferation and induced apoptosis. ORPE treatment with 600 µg/mL extract caused 5.82 % late cell apoptosis and 5.62 % early apoptosis. Cell cycle arrest occurred during G0/G1 phase. Conclusion: ORPE is a potential anticancer agent against human liver cancer cells due to its ability to induce apoptosis of huh7it cells by promoting cell cycle arrest during G0/G1 phase.
AB - Purpose: To examine raw polysaccharides extract of okra (Abelmoschus esculentus L.) as an anticancer agent against human liver cancer cells. Methods: Okra raw polysaccharide extract (ORPE) was obtained by ethanol extraction from the raw fruit. Huh7it cells were grown in DMEM (Dulbecco's Modified Eagle Medium) and cultured for 24 h prior to treatment with the extract. The cell culture was divided into 3 groups, viz, negative control group (KN), positive control group (KP, treated with 10 μg/mL doxorubicin), and ORPE (P) group. ORPE group was divided into 5 subgroups based on the dose used for treatment, viz, 50 (P1), 100 (P2), 200 (P3), 400 (P4), and 600 µg/mL (P5). Huh7it cell proliferation was measured by MTT assay. while measurement of Huh7it cell apoptosis, necrosis, and cell cycle analysis were carried out using Annexin V FITC-PI antibody test and flow cytometry. Results: ORPE significantly inhibited Huh7it cell proliferation and induced apoptosis. ORPE treatment with 600 µg/mL extract caused 5.82 % late cell apoptosis and 5.62 % early apoptosis. Cell cycle arrest occurred during G0/G1 phase. Conclusion: ORPE is a potential anticancer agent against human liver cancer cells due to its ability to induce apoptosis of huh7it cells by promoting cell cycle arrest during G0/G1 phase.
KW - Abelmoschus esculentus L
KW - Anticancer
KW - Apoptosis
KW - Cell cycle
KW - Cell proliferation
KW - Liver cancer
KW - Raw okra polysaccharides
UR - http://www.scopus.com/inward/record.url?scp=85074987444&partnerID=8YFLogxK
U2 - 10.4314/tjpr.v18i8.15
DO - 10.4314/tjpr.v18i8.15
M3 - Article
AN - SCOPUS:85074987444
SN - 1596-5996
VL - 18
SP - 1667
EP - 1672
JO - Tropical Journal of Pharmaceutical Research
JF - Tropical Journal of Pharmaceutical Research
IS - 8
ER -