TY - JOUR
T1 - Antibody Titers in the Sheep which were Immunated Antigen of Whole Protein from Third Instar Larvae Musca domestica
AU - Ariantini, B.
AU - Ratnani, H.
AU - Luqman, E. M.
AU - Hastutiek, P.
N1 - Publisher Copyright:
© 2019 Published under licence by IOP Publishing Ltd.
PY - 2019/1/9
Y1 - 2019/1/9
N2 - This research aimed to know antibody titers in the sheep which were injected antigen of whole protein from third instar larvae Musca domestica by Enzyme-Linked Immunosorbent Assay (ELISA), that giving the antigen could cause increasing antibody titers in the sheep. A male sheep about two years old were immunated with whole protein from larvae Musca domestica which was added Freund's Complete Adjuvant and done three times injection again (booster) with range of time once-two weeks and addition of Freund's Incomplete Adjuvant. Taking sheep blood before and after immunitation was done to get serum as composition of ELISA test. Data which were got in this research, tabulated with descriptive analysis. The result of this research showed that there was a little of increasing antibody titers. The most titers at booster 1, then gradually had drastic decreasing. Based on the result of ELISA test can be concluded that antigen of whole protein from larvae Musca domestica can't stimulate immune response in the sheep.
AB - This research aimed to know antibody titers in the sheep which were injected antigen of whole protein from third instar larvae Musca domestica by Enzyme-Linked Immunosorbent Assay (ELISA), that giving the antigen could cause increasing antibody titers in the sheep. A male sheep about two years old were immunated with whole protein from larvae Musca domestica which was added Freund's Complete Adjuvant and done three times injection again (booster) with range of time once-two weeks and addition of Freund's Incomplete Adjuvant. Taking sheep blood before and after immunitation was done to get serum as composition of ELISA test. Data which were got in this research, tabulated with descriptive analysis. The result of this research showed that there was a little of increasing antibody titers. The most titers at booster 1, then gradually had drastic decreasing. Based on the result of ELISA test can be concluded that antigen of whole protein from larvae Musca domestica can't stimulate immune response in the sheep.
UR - http://www.scopus.com/inward/record.url?scp=85060018098&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/217/1/012022
DO - 10.1088/1755-1315/217/1/012022
M3 - Conference article
AN - SCOPUS:85060018098
SN - 1755-1307
VL - 217
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012022
T2 - 2nd International Conference on Collaboration Seminar of Chemistry and Industry, CoSCI 2018, in conjunction with 23rd Indonesian Society for Biochemistry and Molecular Biology, ISBMB 2018
Y2 - 11 October 2018 through 12 October 2018
ER -