This research aimed to know antibody titers in the sheep which were injected antigen of whole protein from third instar larvae Musca domestica by Enzyme-Linked Immunosorbent Assay (ELISA), that giving the antigen could cause increasing antibody titers in the sheep. A male sheep about two years old were immunated with whole protein from larvae Musca domestica which was added Freund's Complete Adjuvant and done three times injection again (booster) with range of time once-two weeks and addition of Freund's Incomplete Adjuvant. Taking sheep blood before and after immunitation was done to get serum as composition of ELISA test. Data which were got in this research, tabulated with descriptive analysis. The result of this research showed that there was a little of increasing antibody titers. The most titers at booster 1, then gradually had drastic decreasing. Based on the result of ELISA test can be concluded that antigen of whole protein from larvae Musca domestica can't stimulate immune response in the sheep.
|Journal||IOP Conference Series: Earth and Environmental Science|
|Publication status||Published - 9 Jan 2019|
|Event||2nd International Conference on Collaboration Seminar of Chemistry and Industry, CoSCI 2018, in conjunction with 23rd Indonesian Society for Biochemistry and Molecular Biology, ISBMB 2018 - Surabaya, Indonesia|
Duration: 11 Oct 2018 → 12 Oct 2018