TY - JOUR
T1 - Antibiotic susceptibilities and genetic characteristics of extended-spectrum beta-lactamase-producing Escherichia coli isolates from stools of pediatric diarrhea patients in Surabaya, Indonesia
AU - Bagus Wasito, Eddy
AU - Shigemura, Katsumi
AU - Osawa, Kayo
AU - Fardah, Alpha
AU - Kanaida, Akiho
AU - Raharjo, Dadik
AU - Kuntaman, K.
AU - Hadi, Usman
AU - Harijono, Sugeng
AU - Marto Sudarmo, Subijanto
AU - Nakamura, Tatsuya
AU - Shibayama, Keigo
AU - Fujisawa, Masato
AU - Shirakawa, Toshiro
N1 - Publisher Copyright:
© 2017, National Institute of Health. All rights reserved.
PY - 2017
Y1 - 2017
N2 - SUMMARY: The purpose of this study was to investigate extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates from pediatric (aged 0 to 3 years) diarrhea patients in Surabaya, Indonesia, where this kind of survey is rare; our study included assessment of their antibiotic susceptibilities, as well as ESBL typing, multilocus sequence typing (MLST), and diarrheagenic E. coli (DEC)-typing. ESBL-producing E. coli were detected in 18.8% of all the samples. Many ESBL-producing E. coli had significantly lower susceptibility to gentamicin (p < 0.0001) and the quinolones nalidixic acid (p=0.004) and ciprofloxacin (p < 0.0001) than non-producers. In ESBL-producing E. coli, 84.0% of strains expressed CTX-M-15 alone or in combination with other ESBL types. MLST revealed that 24.0% of ESBL-producers had sequence type 617, all of which expressed the CTX-M-15 gene; we also detected expression of 3 DEC-related genes: 2 enteroaggregative E. coli genes and 1 enteropathogenic E. coli gene. In conclusion, CTX-M-15-type ESBL-producing E. coli ST617 appear to have spread to Indonesia.
AB - SUMMARY: The purpose of this study was to investigate extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates from pediatric (aged 0 to 3 years) diarrhea patients in Surabaya, Indonesia, where this kind of survey is rare; our study included assessment of their antibiotic susceptibilities, as well as ESBL typing, multilocus sequence typing (MLST), and diarrheagenic E. coli (DEC)-typing. ESBL-producing E. coli were detected in 18.8% of all the samples. Many ESBL-producing E. coli had significantly lower susceptibility to gentamicin (p < 0.0001) and the quinolones nalidixic acid (p=0.004) and ciprofloxacin (p < 0.0001) than non-producers. In ESBL-producing E. coli, 84.0% of strains expressed CTX-M-15 alone or in combination with other ESBL types. MLST revealed that 24.0% of ESBL-producers had sequence type 617, all of which expressed the CTX-M-15 gene; we also detected expression of 3 DEC-related genes: 2 enteroaggregative E. coli genes and 1 enteropathogenic E. coli gene. In conclusion, CTX-M-15-type ESBL-producing E. coli ST617 appear to have spread to Indonesia.
KW - CTX-M-15
KW - Diarrheagenic Escherichia coli
KW - Extended-spectrum beta-lactamase (ESBL)
KW - ST617
UR - http://www.scopus.com/inward/record.url?scp=85025616009&partnerID=8YFLogxK
U2 - 10.7883/yoken.JJID.2016.234
DO - 10.7883/yoken.JJID.2016.234
M3 - Article
C2 - 28003592
AN - SCOPUS:85025616009
SN - 1344-6304
VL - 70
SP - 378
EP - 382
JO - Japanese Journal of Infectious Diseases
JF - Japanese Journal of Infectious Diseases
IS - 4
ER -