Antibiofilm Activty of Epsilon Polylysine (e-PL) Against Enterococcus Faecalis Biofilm as a Root Canal Dressing Candidate

The primary objective of endodontic treatment is to thoroughly clean, disinfect, shape, as well as seal the canal in three dimensions to eliminate or prevent reinfection. Failure of endodontic therapy are associated with persistently presence of bacteria, microleakage at the crown, inadequately cleaned and filled root canals, as well as missed canals. E. faecalis can survive in dentine tubules within the system, endure challenging conditions like starvation and an alkaline pH, and form biofilms, contributing to its persistence even after root canal treatment was done. Reflecting on these factors, utilizing innovative dressing materials would be beneficial in enhancing the success of endodontic therapy. Antimicrobial peptides specifically Epsilon-polylysine (ε-PL) has recently emerged as an extremely significant novel antibiofilm and serves as intracanal dressing. The aim of this study is to evaluate the antibiofilm activity of Epsilon-Polylysine against Enterococcus faecalis biofilm. This study highlights Enterococcus faecalis biofilm as one of the causes of endodontic failure. There are numerous conventional intracanal medications on the market with its limitations. This review discusses and focus on Epsilon-polylysine, which is a significant novel antimicrobial peptide as well as an intracanal medication. AMPs interact with the surface of microbial membranes or act inside the target cell, displaying a broad spectrum of activity. The low antimicrobial concentration and tissue repair capability of AMPs establish its role as antibiofilm. Epsilon-Polylysine has an antibiofilm activity against Enterococcus faecalis biofilm as indicated in MBIC and MBEC values.