Antibacterial Differences Effect between Purple Leaves (Graptophyllum Pictum (L) Griff.) 70% And 96% Ethanol Extract Against Aggregatibacter Actinomycetemcomittans Bacteria

Since the main causative agent for local aggressive periodontitis was the Aggregatibacter actinomycetemcomitans bacteria (A. actinomycetemcomittans). Purple leaves (Graptophyllum pictum (L) Griff.), which contain antibacterial compounds such as flavonoids, tannins, saponins, steroids, and alkaloids, are chosen as one of the natural medicinal ingredients. However, such antibacterial compounds that contain 70% ethanol and 96% ethanol are different. This study proves the differences between the antibacterial effect of 70% and 96% of ethanol extract of purple leaves against the A. actinomycetemcomitans bacteria. This study, purple leaf Simplicia, was extracted with 70% and 96% ethanol using the maceration method. The antibacterial power test has carried out using the serial dilution method to determine MIC and MBC. The diffusion method on the Luria Bertani media utilized to measure the inhibition zone has formed. It has revealed that there was no significant difference between the number of growths of bacterial colonies on MIC and MBC for 70% and 96% ethanol extract of purple leaves. MIC and MBC in 70% and 96% ethanol extract of purple leaves were 3.125% and 6.25%. Unlike the serial dilution test, the diffusion test obtained a significant difference with p=0.019 (p=<0.05). In ethanol, 96% of the inhibition zone's diameter is 13.13 mm, and in 70% ethanol, is 16.5 mm. In short, 70% and 96% ethanol extract of purple leaves has the same antibacterial effect on A. actinomycetemcomittans bacteria. However, the extra antibacterial activity of 70% ethanol in bacteria is more than the 96% ethanol extract of purple leaves.