TY - JOUR
T1 - Antibacterial activity of 1 % Roselle flower nano-emulsion extract (Hibiscus sabdariffa) against peri-implantitis-related bacteria on orthodontic mini-implants
T2 - An in vitro study
AU - Abdillah, Ridhofar Akbar Khusnul
AU - Budhy, Theresia Indah
AU - Narmada, Ida Bagus
AU - Ardani, I. Gusti Aju Wahju
AU - Ramadhani, Nastiti Faradilla
AU - Sitalaksmi, Ratri Maya
AU - Luthfi, Muhammad
AU - Ulfa, Ninik Mas
AU - Nugraha, Albertus Putera
AU - Tengku Ahmad Noor, Tengku Natasha Eleena binti
AU - Nugraha, Alexander Patera
N1 - Publisher Copyright:
© 2025 The Authors
PY - 2025/1/1
Y1 - 2025/1/1
N2 - Objective: The orthodontic mini-implant (OMI) failure often occurs due to the accumulation of peri-implantitis bacteria surrounding it, which results in a stable, resistant form of absolute skeletal anchorage during orthodontic treatment. Administering doxycycline may be the solution, but long-term side effects result in antibiotic resistance. Roselle flowers (Hibiscus sabdariffa) possess beneficial active phytochemical substances, which may have potential as an OMI peri-implantitis alternative therapy. This study investigates the antibacterial activity of 1 % Roselle flower (H. sabdarifa) nanoemulsion (NE) extract (1 % RNE) toward peri-implantitis bacteria in OMIs. Methods: A phytochemical analysis of 1 % RNE was carried out to examine the active substances possessed in it, such as flavonoids, quinone, saponin, alkaloids, tannins, terpenoids, and steroids. Nanoemulsion characterization was carried out using a particle size analyzer (PSA). The antibacterial activity of 1 % RNE toward Prevotella intermedia (Pi), Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa), and Fusobacterium nucleatum (Fn) was carried out to determine the minimum inhibitory concentration, minimum bactericidal concentration, and inhibitory zone compared with doxycycline as a positive control. Results: Roselle flower NE extract (1 %) possessed flavonoids, quinone, saponin, alkaloids, tannins, terpenoids, and steroids positively. The PSA showed that the 1 % RNE had a size of 98.13 d nm. The antibacterial activity of 1 % RNE against Aa, Pg, Pi, and Fn bacteria at 3.125 % showed significant differences (p < 0.05). The antibacterial activity of 1 % RNE toward peri-implantitis bacteria is lower than that of doxycycline. Conclusion: Roselle flower NE extract (1 %) has antibacterial activity against peri-implantitis bacteria at a concentration of 3.125 %.
AB - Objective: The orthodontic mini-implant (OMI) failure often occurs due to the accumulation of peri-implantitis bacteria surrounding it, which results in a stable, resistant form of absolute skeletal anchorage during orthodontic treatment. Administering doxycycline may be the solution, but long-term side effects result in antibiotic resistance. Roselle flowers (Hibiscus sabdariffa) possess beneficial active phytochemical substances, which may have potential as an OMI peri-implantitis alternative therapy. This study investigates the antibacterial activity of 1 % Roselle flower (H. sabdarifa) nanoemulsion (NE) extract (1 % RNE) toward peri-implantitis bacteria in OMIs. Methods: A phytochemical analysis of 1 % RNE was carried out to examine the active substances possessed in it, such as flavonoids, quinone, saponin, alkaloids, tannins, terpenoids, and steroids. Nanoemulsion characterization was carried out using a particle size analyzer (PSA). The antibacterial activity of 1 % RNE toward Prevotella intermedia (Pi), Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa), and Fusobacterium nucleatum (Fn) was carried out to determine the minimum inhibitory concentration, minimum bactericidal concentration, and inhibitory zone compared with doxycycline as a positive control. Results: Roselle flower NE extract (1 %) possessed flavonoids, quinone, saponin, alkaloids, tannins, terpenoids, and steroids positively. The PSA showed that the 1 % RNE had a size of 98.13 d nm. The antibacterial activity of 1 % RNE against Aa, Pg, Pi, and Fn bacteria at 3.125 % showed significant differences (p < 0.05). The antibacterial activity of 1 % RNE toward peri-implantitis bacteria is lower than that of doxycycline. Conclusion: Roselle flower NE extract (1 %) has antibacterial activity against peri-implantitis bacteria at a concentration of 3.125 %.
KW - Hibiscus sabdarifa
KW - Medicine
KW - Nanoemulsion
KW - Orthodontic
KW - Peri-implantitis
UR - http://www.scopus.com/inward/record.url?scp=85214329119&partnerID=8YFLogxK
U2 - 10.1016/j.jobcr.2024.12.017
DO - 10.1016/j.jobcr.2024.12.017
M3 - Article
AN - SCOPUS:85214329119
SN - 2212-4268
VL - 15
SP - 170
EP - 177
JO - Journal of Oral Biology and Craniofacial Research
JF - Journal of Oral Biology and Craniofacial Research
IS - 1
ER -