Antibacterial activity and phytochemical analysis of ethanolic purple leaf extract (Graptophyllum Pictum L.griff) on lactobacillus acidophilus

Introduction: : Lactobacillus acidophilus is an anaerobic facultative gram-positive bacterium that contributes to secondary caries. A cavity cleanser was needed as an antibacterial agent to eliminate oral microorganism which contaminated the tooth cavity. Graptophyllum pictum L.Griff is one of the herbal medicaments which has the potential to be used as an antibacterial agent as it contains active antibacterial chemical-compound. Methods: Qualitative phytochemical analysis of purple leaves extract was determined with several methods. Lactobacillus acidophilus ATCC 4356 was suspended into several concentrations of Graptophyllum pictum L.Griff extract from the dilution on BHIB medium. Results: Purple leaves extract contained some bioactive compounds flavonoid, alkaloid, tannin, triterpenoid/steroid, and saponin. Minimum Inhibitory Concentration (MIC) of the ethanolic purple leaves extract was 6.25% and Minimum Bactericidal Concentration (MBC) of the ethanolic purple leaf extract was 12.5% against Lactobacillus acidophilus. Conclusion: Ethanolic purple leaves extract (Graptophyllum pictum L.Griff) has antibacterial activity against Lactobacillus acidophilus.