TY - JOUR
T1 - Antiangiogenesis activity test of tin leaf (Ficus carica L.) on the number of blood vessels and VEGF expression of chorioallantoic membrane of embryonated chicken eggs
AU - Hamid, Iwan Sahrial
AU - Aksono, Eduardus Bimo
AU - Sukmanadi, Mohammad
AU - Purnama, Muhammad Thohawi Elziyad
N1 - Publisher Copyright:
Copyright © 2018 the Author(s). Published by Wolters Kluwer Health, Inc.
PY - 2019
Y1 - 2019
N2 - This research has been conducted on the antiangiogenesis activity test of the tin leaf (Ficus carica L.) on the number of blood vessels and vascular endothelial growth factor (VEGF) expression of chorioallantoic membrane (CAM) of embryonated chicken eggs. The general objective of this study is the use of natural products from the F. carica L. as chemopreventive in cancer through the mechanism of inhibition of angiogenesis. Inhibition of angiogenesis, supply of nutrients and oxygen to cancer cells will also be inhibited, so that indirectly cancer cells will be stunted. This study used 25 embryonated chicken eggs that divided into 5 treatments and each treatment has 5 repetitions. This treatment includes placebo negative controls, celecoxib-positive controls, and 3 treatment groups were given a water extract of F. carica L. which was divided into several doses of 75, 90, and 110 mg. The result found a significant difference (P < .05) in the macroscopic observation of the number of blood vessels in the CAM loaded with the paper dish. Observation of VEGF expression in vascular endothelial cells in the CAM also had a significant difference (P < .05). The optimal dose of water extract of F. carica L. of 90 mg has been able to inhibit the formation of new blood vessels by 65.51% and reduce VEGF expression by 45% in the chorioallantoic membrane. Thus, it can be concluded that the administration of water extract of F. carica L. can inhibit the formation of new blood vessels and inhibit VEGF expression.
AB - This research has been conducted on the antiangiogenesis activity test of the tin leaf (Ficus carica L.) on the number of blood vessels and vascular endothelial growth factor (VEGF) expression of chorioallantoic membrane (CAM) of embryonated chicken eggs. The general objective of this study is the use of natural products from the F. carica L. as chemopreventive in cancer through the mechanism of inhibition of angiogenesis. Inhibition of angiogenesis, supply of nutrients and oxygen to cancer cells will also be inhibited, so that indirectly cancer cells will be stunted. This study used 25 embryonated chicken eggs that divided into 5 treatments and each treatment has 5 repetitions. This treatment includes placebo negative controls, celecoxib-positive controls, and 3 treatment groups were given a water extract of F. carica L. which was divided into several doses of 75, 90, and 110 mg. The result found a significant difference (P < .05) in the macroscopic observation of the number of blood vessels in the CAM loaded with the paper dish. Observation of VEGF expression in vascular endothelial cells in the CAM also had a significant difference (P < .05). The optimal dose of water extract of F. carica L. of 90 mg has been able to inhibit the formation of new blood vessels by 65.51% and reduce VEGF expression by 45% in the chorioallantoic membrane. Thus, it can be concluded that the administration of water extract of F. carica L. can inhibit the formation of new blood vessels and inhibit VEGF expression.
KW - Angiogenesis
KW - Chorioallantoic membrane
KW - Vascular endothelial growth factor expression
UR - http://www.scopus.com/inward/record.url?scp=85074122355&partnerID=8YFLogxK
U2 - 10.1097/OP9.0000000000000007
DO - 10.1097/OP9.0000000000000007
M3 - Article
AN - SCOPUS:85074122355
SN - 1783-3914
VL - 1
JO - European Journal of Oncology Pharmacy
JF - European Journal of Oncology Pharmacy
IS - 4
M1 - e7
ER -