Anti Immunogenicity Evaluation of Bovine Demineralized Dentine Membrane Material

Introduction: Proliferation of fibroblasts in soft tissue occurs relatively faster than hard tissue's regeneration. For this reason, Guided Bone regeneration (GBR) is needed as a barrier to prevent any interventions between both processes in bone regeneration. Demineralized Dentine Material Membrane (DDMM) is a new material as GBR that must be ensured to have good properties. and do not cause a reaction in human body. Some of the influencing factors are still unspecified, which makes it difficult to expect biological respons, for example anti immunogenicity, which could lead to severe side effects. Methods: Three DDMM samples were performed by HE staining to see whether there were any residual cells or material. DDMM placed on the object glass. HE staining was stained. The HE method was selected according to the purpose of the study. Microscopic analysis was carried out through the division of the visual field into nine sections. each section is examined, to find the remaining odontoblast cells in the area. Results: the entire microscope field of view of the DDMM sample contained mineralized dentin material, with no odontoblast cell remaining. DDMM slices on processing step do not affect the pulp wall. dentine tubules appear to be cut with transverse direction. Conclusion: DDMM does not contain any cells or chemical structure that may caused an immunogenic reaction.