Anti-carcinogenic activity of Gynura procumbens extract through cytochrome P450 and glutathione S-transferase

This study aim to examine the molecular activity of cytochrome P450 and enzymatic GSTμ (glutathione S-transferase μ) in Sprague-Dawley rat liver after induction of G. procumbens extract. Sprague Dawley female rats aged 40 days were divided into 5 groups, group I was the positive control which treated with 7,12-Dimethylbenz(a)anthracene (DMBA) 20 mg / kg bw; group II and III were given ethanol extract of G. procumbens 300 mg / kg bw and 750 mg / kg bw DMBA plus 20 mg / kg bw; group IV and V is a negative control which treated with the extract of 300 and 750 mg / kg bw without DMBA administration. In the third week of treatment, rat hepatic cytochrome P4501A1 activity was measured using the substrate 7ethoxyresorufin-O-deethylase (EROD), while GSTμ reacted with 1-Chloro-2,4-dinitrobenzene measured by spectrophotometry λ 340 nm. The results showed significant decrement of cytochrome P450 activity (p <0.05) and the significant increasing of GSTμ activity (p <0.05) in rat which given G. procumbens extract at concentration 300 and 750 mg / kg bw. In conclusion, the ethanol extract of G. procumbens leaf acts as a blocking agent in carcinogenesis initiation phase which inhibits further progression towards malignancy.