Development of andrographolide-carboxymethyl chitosan nanoparticles: Characterization, in vitro release and in vivo antimalarial activity study

Retno Sari, Aty Widyawaruyanti, Franciscus B.Tedy Anindita, Sinta Kusuma Astuti, Dwi Setyawan

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Objectives: The purpose of this study was to investigate the effect of andrographolide-carboxymethyl chitosan nanoparticles formation on the physical characteristics, in vitro release profile and in vivo antimalarial activity of andrographolide. Materials and Methods: Nanoparticles were prepared by ionic gelation method-spray drying using CaCl2 as the crosslinker with a composition of drug: polymer: CaCl2=40: 250: 100. The obtained particles were evaluated for its size and morphology; physical state, drug content, in vitro drug release and in vivo antimalarial activity on Plasmodium berghei infected mice. Results: The results of DTA and XRD showed that nanoparticle systems had a lower melting point and lower crystallinity degree. The drug dissolved from the nanoparticles was increased up to 6.5 times and the in vivo antimalarial activity was 1.65 times higher compared to andrographolide. Conclusion: The formation andrographolide-carboxymethyl chitosan nanoparticles affected the physical characteristics of andrographolide. The decrease crystallinity of andrographolide resulted in a lower melting point of andrographolide. Such changes provided a positive impact to the drug dissolution and then its activity.

Original languageEnglish
Pages (from-to)136-141
Number of pages6
JournalTurkish Journal of Pharmaceutical Sciences
Volume15
Issue number2
DOIs
Publication statusPublished - Aug 2018

Keywords

  • Andrographolide
  • Carboxymethyl chitosan
  • In vitro release
  • In vivo antimalarial
  • Ionic gelation
  • Spray drying

Fingerprint

Dive into the research topics of 'Development of andrographolide-carboxymethyl chitosan nanoparticles: Characterization, in vitro release and in vivo antimalarial activity study'. Together they form a unique fingerprint.

Cite this