@article{8d3e4bd66d16435fba831be2ef5c96b7,
title = "Analysis of the upstream genetic structures of the ISEcp1-blaCTX-M transposition units in Escherichia coli isolates carrying blaCTX-M obtained from the Indonesian and Vietnamese communities",
abstract = "Extended spectrum β-lactamase (ESBL)-producing Escherichia coli have been found in healthy individuals in Indonesia and Vietnam. The ISEcp1-blaCTX-M transposition unit of ESBL-producing bacterial isolates has been considered responsible for the production of CTX-M type ESBL and it is important for the dissemination of blaCTX-M. This study aimed to characterize the upstream genetic structure (UGS) of E. coli isolates possessing blaCTX-M-1 group and/or blaCTX-M-9 group genes obtained from healthy individuals in Indonesia and Vietnam. A total of 501 CTX-M type ESBL-producing E. coli isolates possessing blaCTX-M-1 group and/or blaCTX-M-9 group genes were obtained from healthy individuals of the two countries in 2018. The UGSs of the ISEcp1-blaCTX-M transposition unit of the 501 ESBL-producing E. coli isolates were amplified by barcode-adaptor-ligation-mediated PCR and analyzed using the Nanopore sequencer. The obtained sequence information was used to classify the UGSs of the ISEcp1-blaCTX-M transposition unit. From the 501 ESBL-producing E. coli isolates, 502 UGSs were obtained, which were classified into 85 UGS types based on the sequence. ISEcp1 of 359 (71.5%) of the 502 UGSs was disrupted by gene insertion, and ISEcp1-blaCTX-M transposition unit of most (87.1%) of the determined UGSs was confirmed as plasmidic. Only 6 (7.1%) of the 85 UGS types were common to both countries. Our results indicated that many different UGSs of ISEcp1-blaCTX-M transposition units were detected in Indonesia and Vietnam; hence, we suggest that structurally different kinds of plasmids harboring blaCTX-M were separately distributed in the two countries.",
keywords = "ESBL-producingEscherichia coli, ISEcp1-bla, antimicrobial resistance, molecular epidemiology, nanopore sequencing",
author = "Widyatama, {Fikri S.} and Nobuyoshi Yagi and Rosantia Sarassari and Toshiro Shirakawa and Le, {Danh Tuyen} and Bui, {Mai Huong Thi} and Kuntaman Kuntaman and Itaru Hirai",
note = "Funding Information: We would like to thank past and present members of Department of Clinical Microbiology, Dr Soetomo Hospital – Faculty of Medicine, Airlangga University, Surabaya, Indonesia and of University of the Ryukyus for supporting this study. DNA sequencing analysis was performed at the University of the Ryukyus Center for Research Advancement and Collaboration. This work was partly supported by Japan Agency for Medical Research and Development (AMED), Japan/Japan International Cooperation Agency (JICA), Japan as part of the Science and Technology Research Partnership for Sustainable Development (SATREPS), the e‐ASIA Joint Research Program (e‐ASIA JR), AMED, Japan and Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number 17H04663 and JSPS KAKENHI Grant Number 17J08848. Funding Information: We would like to thank past and present members of Department of Clinical Microbiology, Dr Soetomo Hospital – Faculty of Medicine, Airlangga University, Surabaya, Indonesia and of University of the Ryukyus for supporting this study. DNA sequencing analysis was performed at the University of the Ryukyus Center for Research Advancement and Collaboration. This work was partly supported by Japan Agency for Medical Research and Development (AMED), Japan/Japan International Cooperation Agency (JICA), Japan as part of the Science and Technology Research Partnership for Sustainable Development (SATREPS), the e-ASIA Joint Research Program (e-ASIA JR), AMED, Japan and Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number 17H04663 and JSPS KAKENHI Grant Number 17J08848. Publisher Copyright: {\textcopyright} 2021 The Societies and John Wiley & Sons Australia, Ltd.",
year = "2021",
month = dec,
doi = "10.1111/1348-0421.12938",
language = "English",
volume = "65",
pages = "542--550",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Wiley-Blackwell",
number = "12",
}