Analysis of the density and intensity of metallothionein in Crassostrea cucullata oyster hulls in the coastal fishing port of Mayangan Probolinggo, East Java using immunohistochemical techniques

The purpose of this study was to determine the relationship between heavy metal levels in the stomach of Crassostrea cucullata. Descriptive methods and oyster sampling were carried out at 3 stations and 3 oyster samples were taken from each station. Station I, it is suspected that the pollution came from waste from ship engine oil, or waste from ship repair, Station II is suspected of having contamination originating from TPI or SPN. Station III, it is suspected that the pollution came from domestic waste from the community who used the river as a disposal site. The results of the metalothionein density are in accordance with the response of the oyster hull to heavy metal absorption which indicates that the heavy metal content in the stomach at station 1 is higher when compared to station 2 and station 3. The highest metalothionein intensity is found at station 1 which is the dock area, while the lowest metalothionein intensity is found. located at station 1. station 3, namely the mangrove area. The color intensity of metalothionein varies at each station depending on the absorption rate of heavy metals by the oyster body. Average Metallothionein Intensity Graph.