TY - JOUR
T1 - Analysis of the core and E1 envelope region sequences of a novel variant of hepatitis C virus obtained in Indonesia
AU - Hotta, H.
AU - Doi, H.
AU - Hayashi, T.
AU - Purwanta, M.
AU - Soemarto, W.
AU - Mizokami, M.
AU - Ohba, K.
AU - Homma, M.
PY - 1994/3
Y1 - 1994/3
N2 - Hepatitis C virus (HCV) is currently classified into 6 major types, HCV-1 through -6, each of which can be further divided into a few subtypes, e.g., HCV-1a, -1b, -1c, etc., on the basis of sequence variation of the viral genome. The core and E1 envelope regions of HCV genome were amplified from sera of Indonesian patients using reverse transcription-polymerase chain reaction. Sequence analysis of both core and E1 regions followed by molecular evolutionary phylogenetic analysis identified a novel sequence variant of HCV-1 (Td-6). Antibodies in the serum from which Td-6 was isolated reacted only marginally to the core protein of HCV-J, a representative strain of HCV-1b, despite strong antibody response against a mixture of the core, NS3 and NS4 proteins of HCV-1a. The possible mechanism for the diminished reactivity of the antibodies in the serum to the core protein of HCV-J is discussed.
AB - Hepatitis C virus (HCV) is currently classified into 6 major types, HCV-1 through -6, each of which can be further divided into a few subtypes, e.g., HCV-1a, -1b, -1c, etc., on the basis of sequence variation of the viral genome. The core and E1 envelope regions of HCV genome were amplified from sera of Indonesian patients using reverse transcription-polymerase chain reaction. Sequence analysis of both core and E1 regions followed by molecular evolutionary phylogenetic analysis identified a novel sequence variant of HCV-1 (Td-6). Antibodies in the serum from which Td-6 was isolated reacted only marginally to the core protein of HCV-J, a representative strain of HCV-1b, despite strong antibody response against a mixture of the core, NS3 and NS4 proteins of HCV-1a. The possible mechanism for the diminished reactivity of the antibodies in the serum to the core protein of HCV-J is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0028010470&partnerID=8YFLogxK
U2 - 10.1007/BF01538816
DO - 10.1007/BF01538816
M3 - Article
C2 - 7545932
AN - SCOPUS:0028010470
SN - 0304-8608
VL - 136
SP - 53
EP - 62
JO - Archives of Virology
JF - Archives of Virology
IS - 1-2
ER -