Abstract
Introduction: Leucocytozoonosis is a protozoan disease in chickens with high mortality and is an endemic in Indonesia. The aims of this research to analyze the immune response of humoral (IgG) and cellular (CD4) in rabbits immunized by the antigenic proteins of L. caulleryi. Methods: The research were done: separation and extraction of antigenic proteins of L. caulleryi from liver chicken, immunize rabbits with antigenic proteins, detection of IgG titers with indirect ELISA, and detection of CD4 expression with Immunocytochemistry, and visualized by fluorescein isothiocyanate with fluorescence microscope. Results: The immunization results indicate that the protein from the liver contains L. caulleryi which is antigenic and has ability to produce and increase the antibody titers (IgG) of rabbits at a dose of 500 μg per booster. Activation cellular response (CD4) was shown a greenish color by fluorescence microscopy on the fifth booster. Conclusion: The antigenic proteins of L. caulleryi dose of 500 μg with 5 times booster can induce a humoral (IgG) and cellular immune response (CD4) in the fifth booster or 10 weeks after the rabbit is immunized.
| Original language | English |
|---|---|
| Pages (from-to) | 41-43 |
| Number of pages | 3 |
| Journal | Malaysian Journal of Medicine and Health Sciences |
| Volume | 17 |
| Publication status | Published - Apr 2021 |
Keywords
- CD4
- IgG
- Leucocytozoon caulleryi
- Rabbits