TY - JOUR
T1 - Analysis of recombinant, multivalent dengue virus containing envelope (E) proteins from serotypes-1, -3 and -4 and expressed in baculovirus
AU - Rantam, Fedik A.
AU - Purwati,
AU - Soegijanto, S.
AU - Susilowati, H.
AU - Sudiana, K.
AU - Hendrianto, E.
AU - Soetjipto,
N1 - Publisher Copyright:
© 2013 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-NDlicense.
PY - 2015
Y1 - 2015
N2 - Dengue virus has four serotypes that cause a public health problem in Indonesia. Currently, there is no preventative vaccine for this disease, but some model vaccines are in development. The envelop (E) protein genes from three isolates of dengue virus (DENV-1, -3 and -4) were isolated, cloned into Escherichia coli and then sub-cloned into a baculovirus vector before co-transfection into Sf9 cells. Recombinant E genes were inserted between the Smal and Sacl sites of the plasmid, adjacent to the baculoviral structural gene, polyhedrin. The sequence of recombinant E gene was relatively stable with 97-98% homology, although there were amino acid substitutions in some regions. The recombinant protein was more antigenic when exposed to polyclonal sera from infected humans than sera from immunized mice, but its binding to monoclonal antibodies IgG1a and IgG2b was stronger than other isotopes, including IgM, IgG and Ig1b. Recombinant E protein induced cellular immune responses in immunized mice, as demonstrated by lymphocyte secretion of IL-3. This study indicates that recombinant E protein expressed in a baculovirus system can induce humoral and cellular immune responses.
AB - Dengue virus has four serotypes that cause a public health problem in Indonesia. Currently, there is no preventative vaccine for this disease, but some model vaccines are in development. The envelop (E) protein genes from three isolates of dengue virus (DENV-1, -3 and -4) were isolated, cloned into Escherichia coli and then sub-cloned into a baculovirus vector before co-transfection into Sf9 cells. Recombinant E genes were inserted between the Smal and Sacl sites of the plasmid, adjacent to the baculoviral structural gene, polyhedrin. The sequence of recombinant E gene was relatively stable with 97-98% homology, although there were amino acid substitutions in some regions. The recombinant protein was more antigenic when exposed to polyclonal sera from infected humans than sera from immunized mice, but its binding to monoclonal antibodies IgG1a and IgG2b was stronger than other isotopes, including IgM, IgG and Ig1b. Recombinant E protein induced cellular immune responses in immunized mice, as demonstrated by lymphocyte secretion of IL-3. This study indicates that recombinant E protein expressed in a baculovirus system can induce humoral and cellular immune responses.
KW - Dengue virus
KW - Envelope gene
KW - Multivalent
KW - Recombinant E protein
UR - http://www.scopus.com/inward/record.url?scp=84947020587&partnerID=8YFLogxK
U2 - 10.1016/j.trivac.2013.10.001
DO - 10.1016/j.trivac.2013.10.001
M3 - Article
AN - SCOPUS:84947020587
SN - 1879-4378
VL - 4
SP - e75-e79
JO - Trials in Vaccinology
JF - Trials in Vaccinology
ER -
